Unknown

Dataset Information

0

Ilimaquinone Induces Apoptosis and Autophagy in Human Oral Squamous Cell Carcinoma Cells.


ABSTRACT: In this study, the anti-tumor activity of ilimaquinone (IQ), a sesquiterpene quinone isolated from marine sponge Halichondria sp., in oral squamous cell carcinoma (OSCC) cells, was investigated. IQ suppressed the viability of the OSCC cell lines SCC4 and SCC2095 with IC50 values of 7.5 and 8.5 ?M, respectively. Flow cytometric analysis demonstrated that IQ induced caspase-dependent apoptosis in SCC4 cells and modulated the expression of several cell growth-related gene products, including Akt, p38, Mcl-1, and p53. Notably, p53 knockdown caused higher resistance to IQ's anti-tumor activity. In addition, IQ increased reactive oxygen species generation, which was partially reversed by the addition of antioxidants. Furthermore, it triggered autophagy, as evidenced by acidic organelle formation and LC3B-II and Atg5 expression in SCC4 cells. Pretreatment with the autophagy inhibitor 3-methyladenine or chloroquine partially decreased IQ-induced apoptosis, suggesting that IQ induced protective autophagy. In summary, IQ has potential to be used in OSCC therapy.

SUBMITTER: Lin CW 

PROVIDER: S-EPMC7555415 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Ilimaquinone Induces Apoptosis and Autophagy in Human Oral Squamous Cell Carcinoma Cells.

Lin Cheng-Wen CW   Bai Li-Yuan LY   Su Jui-Hsin JH   Chiu Chang-Fang CF   Lin Wei-Yu WY   Huang Wei-Ting WT   Shih Ming-Cheng MC   Huang Yu-Ting YT   Hu Jing-Lan JL   Weng Jing-Ru JR  

Biomedicines 20200820 9


In this study, the anti-tumor activity of ilimaquinone (IQ), a sesquiterpene quinone isolated from marine sponge <i>Halichondria</i> sp., in oral squamous cell carcinoma (OSCC) cells, was investigated. IQ suppressed the viability of the OSCC cell lines SCC4 and SCC2095 with IC<sub>50</sub> values of 7.5 and 8.5 μM, respectively. Flow cytometric analysis demonstrated that IQ induced caspase-dependent apoptosis in SCC4 cells and modulated the expression of several cell growth-related gene products  ...[more]

Similar Datasets

| S-EPMC10002565 | biostudies-literature
| S-EPMC9503335 | biostudies-literature
| S-EPMC7160615 | biostudies-literature
| S-EPMC3553079 | biostudies-other
| S-EPMC5514089 | biostudies-literature
| S-EPMC7011320 | biostudies-literature
| S-EPMC6862293 | biostudies-literature
| S-EPMC11322550 | biostudies-literature
| S-EPMC3299600 | biostudies-literature
| S-EPMC4857543 | biostudies-other