Dataset Information

Combined Microbial Consortium Inoculation and Black Locust Planting Is Effective in the Bioremediation of Waste Drill Cuttings.

ABSTRACT: Waste drill cuttings (WDCs), produced during gas and oil drilling consisting of 80% rock cuttings and 20% drilling muds, are an increasingly potent source of environmental pollution. We studied the efficiency of bioaugmentation and phytoremediation to remediate WDCs in an experiment where WDCs were incubated in a greenhouse for 120 days with and without black locust (Robinia pseudoacacia) plant and with or without bacterial and fungal consortium inoculant. The pollutant removal rates were highest in inoculated and planted treatment, followed by inoculated treatment and planted treatment. The small decrease in contaminant level in the control treatment suggested that indigenous microorganisms in WDCs had little pollutant degradation capability. In the inoculated and planted treatments, after 120 days, the germination rate of red clover seeds was on the same level as in the natural soil, showing a marked decrease in the ecotoxicity of WDC. Both the bacterial and fungal richness and bacterial diversity increased in all the treatments over time, whereas fungal diversity increased only in the not-inoculated treatments. The activity of laccase enzyme played a key role in the bioremediation process. The enzyme activities were mostly governed by inoculated consortium and soil bacterial community, and black locust affected the bioremediation mainly through its effect on N content that further affected bacterial and fungal communities.

SUBMITTER: Liu H

PROVIDER: S-EPMC7555700 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Combined Microbial Consortium Inoculation and Black Locust Planting Is Effective in the Bioremediation of Waste Drill Cuttings.

Liu Hanjun H Chen Lirong L Wang En T ET Liu Yihao Y Zhang Lingzi L Zhao Ke K Gu Yunfu Y Yu Xiumei X Ma Menggen M Penttinen Petri P Zhang Xiaoping X Huang Min M Deng Liangji L Chen Qiang Q

Frontiers in microbiology 20200930

Waste drill cuttings (WDCs), produced during gas and oil drilling consisting of 80% rock cuttings and 20% drilling muds, are an increasingly potent source of environmental pollution. We studied the efficiency of bioaugmentation and phytoremediation to remediate WDCs in an experiment where WDCs were incubated in a greenhouse for 120 days with and without black locust (<i>Robinia pseudoacacia</i>) plant and with or without bacterial and fungal consortium inoculant. The pollutant removal rates were ...[more]

PMID: 33101222

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Project description:Drill cuttings from petroleum exploration and production sites can cause diverse environmental problems. Total petroleum hydrocarbons (TPHs) are a major pollutant from the use of polyolefin-based mud. As an alternative to incineration, this study investigated the application of surfactant-enhanced washing technology prior to bioremediation. The washing step was necessary because the initial TPH concentrations were quite high at approximately 15% (w/w). Washing agents were formulated by varying the concentration of lipopeptide biosurfactant (in foamate or cell-free broth), Dehydol LS7TH (fatty alcohol ethoxylate 7EO, oleochemical surfactant) and butanol (as a lipophilic linker) at different salinities. The most efficient formula produced a Winsor Type I microemulsion (oil-in-water microemulsion) with polyolefin and contained only 20% (v/v) foamate and 2% (v/v) Dehydol LS7TH in water. Due to the synergistic behavior between the anionic lipopeptides and non-ionic Dehydol LS7TH, the formula efficiently removed 92% of the TPHs from the drill cuttings when applied in a jar test. To reduce the cost, the concentrations of each surfactant should be reduced; thus, the formula was optimized by the simplex lattice mixture design. In addition, cell-free broth, at a pH of 10, containing 3.0 g/L lipopeptides was applied instead of foamate because it was easy to prepare. The optimized formula removed 81.2% of the TPHs and contained 72.0% cell-free broth and 1.4% Dehydol LS7TH in water. A 20-kg soil washing system was later tested where the petroleum removal efficiency decreased to 70.7% due to polyolefin redeposition during separation of the washing solution. The remaining TPHs (4.5%) in the washed drilled cuttings were further degraded by a mixture of Marinobacter salsuginis RK5, Microbacterium saccharophilum RK15 and Gordonia amicalis JC11. To promote TPH biodegradation, biochar and fertilizer were applied along with bacterial consortia in a microcosm experiment. After 49-day incubation, the TPHs were reduced to 0.9% by both physical and biological mechanisms, while the TPHs in the unamended samples remained unaffected. With the use of the formulated bio-based washing agent and bioremediation approach, the on-site treatment of drill cuttings could be conducted with an acceptable cost and low environmental impacts.

Project description:As hotspots of local biodiversity in the deep sea, preservation of cold-water coral reef communities is of great importance. In European waters the most extensive reefs are found at depths of 300 - 500 m on the continental margin. In Norwegian waters many of these reefs are located in areas of interest for oil and gas exploration and production. In this study drilling was carried out in the Morvin drill field in proximity to a number of small Lophelia pertusa coral reefs (closest reefs 100 m upstream and 350 m downstream of point of waste drill material release). In a novel monitoring study, ROV video surveys of 9 reefs were conducted prior, during, immediately after and >1 year after drilling operations. Behavior of coral polyps inhabiting reefs exposed to differing concentrations of drill cuttings and drilling fluids (waste drilling material) were compared. Levels of expected exposure to these waste materials were determined for each reef by modelling drill cutting transport following release, using accurate in-situ hydrodynamic data collected during the drilling period and drill cutting discharge data as parameters of a dispersal model. The presence / absence of associate reef species (Acesta excavata, Paragorgia arborea and Primnoa resedaeformis) were also determined from each survey video. There were no significant differences in Lophelia pertusa polyp behavior in corals modelled to have been exposed to pulses of >25 ppm drill cutting material and those modelled to be exposed to negligible concentrations of material. From the video data collected, there were no observed degradations of reef structure over time, nor reductions of associate fauna abundance, regardless of modelled exposure concentration at any of the surveyed reefs. This study focused exclusively on adult fauna, and did not assess the potential hazard posed by waste drilling material to coral or other larvae. Video data was collected by various ROV's, using different camera and lighting setups throughout the survey campaign, making comparison of observations prior, during and post drilling problematic. A standardization of video monitoring in future monitoring campaigns is recommended.

Dataset Information

Combined Microbial Consortium Inoculation and Black Locust Planting Is Effective in the Bioremediation of Waste Drill Cuttings.

Publications

Combined Microbial Consortium Inoculation and Black Locust Planting Is Effective in the Bioremediation of Waste Drill Cuttings.

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