Project description:Sub-Saharan Africa represents 69% of the total number of individuals living with HIV infection worldwide and 72% of AIDS deaths globally. Pulmonary infection is a common and frequently fatal complication, though little is known regarding the lower airway microbiome composition of this population. Our objectives were to characterize the lower airway microbiome of Ugandan HIV-infected patients with pneumonia, to determine relationships with demographic, clinical, immunological, and microbiological variables and to compare the composition and predicted metagenome of these communities to a comparable cohort of patients in the US (San Francisco). Bronchoalveolar lavage samples from a cohort of 60 Ugandan HIV-infected patients with acute pneumonia were collected. Amplified 16S ribosomal RNA was profiled and aforementioned relationships examined. Ugandan airway microbiome composition and predicted metagenomic function were compared to US HIV-infected pneumonia patients. Among the most common bacterial pulmonary pathogens, Pseudomonas aeruginosa was most prevalent in the Ugandan cohort. Patients with a richer and more diverse airway microbiome exhibited lower bacterial burden, enrichment of members of the Lachnospiraceae and sulfur-reducing bacteria and reduced expression of TNF-alpha and matrix metalloproteinase-9. Compared to San Franciscan patients, Ugandan airway microbiome were significantly richer, and compositionally distinct with predicted metagenomes that encoded a multitude of distinct pathogenic pathways e.g secretion systems. Ugandan pneumonia-associated airway microbiome is compositionally and functionally distinct from those detected in comparable patients in developed countries, a feature which may contribute to adverse outcomes in this population. Please note that the data from the comparable cohort of patients in the USUS data was published as supplemental material of PMID: 22760045 but not submitted to GEO The 'patient_info.txt' contains 12 clinical, 7 immunological and 3 microbiological variables for each patient.

Project description:Sub-Saharan Africa represents 69% of the total number of individuals living with HIV infection worldwide and 72% of AIDS deaths globally. Pulmonary infection is a common and frequently fatal complication, though little is known regarding the lower airway microbiome composition of this population. Our objectives were to characterize the lower airway microbiome of Ugandan HIV-infected patients with pneumonia, to determine relationships with demographic, clinical, immunological, and microbiological variables and to compare the composition and predicted metagenome of these communities to a comparable cohort of patients in the US (San Francisco). Bronchoalveolar lavage samples from a cohort of 60 Ugandan HIV-infected patients with acute pneumonia were collected. Amplified 16S ribosomal RNA was profiled and aforementioned relationships examined. Ugandan airway microbiome composition and predicted metagenomic function were compared to US HIV-infected pneumonia patients. Among the most common bacterial pulmonary pathogens, Pseudomonas aeruginosa was most prevalent in the Ugandan cohort. Patients with a richer and more diverse airway microbiome exhibited lower bacterial burden, enrichment of members of the Lachnospiraceae and sulfur-reducing bacteria and reduced expression of TNF-alpha and matrix metalloproteinase-9. Compared to San Franciscan patients, Ugandan airway microbiome were significantly richer, and compositionally distinct with predicted metagenomes that encoded a multitude of distinct pathogenic pathways e.g secretion systems. Ugandan pneumonia-associated airway microbiome is compositionally and functionally distinct from those detected in comparable patients in developed countries, a feature which may contribute to adverse outcomes in this population. Please note that the data from the comparable cohort of patients in the USUS data was published as supplemental material of PMID: 22760045 but not submitted to GEO The 'patient_info.txt' contains 12 clinical, 7 immunological and 3 microbiological variables for each patient. The G2 PhyloChip microarray platform (commercially available from Second Genome, Inc.) was used to profile bacteria in lower airway samples from 60 subjects

Project description:BACKGROUND:Obesity in childhood is associated with adverse health outcomes and complications throughout the life-span of a child. Available evidence suggests a dramatic increase in childhood obesity in sub-Saharan Africa (SSA) over the past two decades. The health risks associated with obesity/overweight are particularly problematic in children due to the potential for long-term health concerns. The researchers propose to conduct a systematic scoping review to map evidence on the burden and distribution of childhood obesity in SSA. METHODS:The study will be guided by the scoping review framework proposed by Arksey and O'Malley. A comprehensive literature search will be performed in the following electronic databases: PubMed, Web of Science, African Index Medicus, and CINAHL with full text via EBSCOhost platform. Primary studies both published in peer-reviewed journals and gray literature such as unpublished studies, thesis, and studies in press addressing the research topic will be included. One reviewer will conduct title screening, and the results will be exported to Mendeley Desktop library. Two independent reviewers will perform both abstract and full article screening in parallel as well as data extraction from eligible studies. The Preferred Reporting Items for Systematic Reviews and Meta-analysis: Extension for Scoping Review (PRISMA-ScR) will be utilized to present the study findings of the proposed scoping review. NVivo version 11.0 will be used to extract the relevant outcomes from the included studies, a content thematic analysis performed, and the results reported using a narrative approach. The Mixed Method Quality Appraisal Tool Version 2018 will be used to assess the methodological quality of all included studies. DISCUSSION:We anticipate that the proposed study will contribute to the existing body of knowledge on childhood obesity, identify gaps in knowledge on the topic, inform future research direction, and provide evidence-based information to strengthen health systems and policies on childhood obesity towards achieving the WHO global target of halting the rise in obesity by 2025.

Dataset Information

Mapping the Geographic Distribution of Tungiasis in Sub-Saharan Africa.

Publications

Mapping the Geographic Distribution of Tungiasis in Sub-Saharan Africa.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets