Transposon Insertion in the purL Gene Induces Biofilm Depletion in Escherichia coli ATCC 25922.

ABSTRACT: Current Escherichia coli antibiofilm treatments comprise a combination of antibiotics commonly used against planktonic cells, leading to treatment failure. A better understanding of the genes involved in biofilm formation could facilitate the development of efficient and specific new antibiofilm treatments. A total of 2578 E. coli mutants were generated by transposon insertion, of which 536 were analysed in this study. After sequencing, Tn263 mutant, classified as low biofilm-former (LF) compared to the wild-type (wt) strain (ATCC 25922), showed an interruption in the purL gene, involved in the de novo purine biosynthesis pathway. To elucidate the role of purL in biofilm formation, a knockout was generated showing reduced production of curli fibres, leading to a

SUBMITTER: Cepas V 

PROVIDER: S-EPMC7558270 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature