Browse
Submit Data
Databases
API
Help

Dataset Information

26 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Draft Genome Sequences of Two Phylogenetically Distinct Clostridium gasigenes Strains, CM001 and CM004, Isolated from Chilled Vacuum-Packed Meat.

ABSTRACT: We present the draft genome sequences of Clostridium gasigenes strains CM001 and CM004. The genomes are 4,147,089 and 4,191,074?bp with GC contents of 28.7% and 28.8%, respectively. Although both strains belong to the same species, whole-genome sequence-based analyses reveal that the strains are phylogenetically distinct.

SUBMITTER: Wambui J

PROVIDER: S-EPMC7561699 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Draft Genome Sequences of Two Phylogenetically Distinct Clostridium gasigenes Strains, CM001 and CM004, Isolated from Chilled Vacuum-Packed Meat.

Wambui Joseph J Cernela Nicole N Stevens Marc J A MJA Corti Sabrina S Stephan Roger R

Microbiology resource announcements 20201015 42

We present the draft genome sequences of <i>Clostridium gasigenes</i> strains CM001 and CM004. The genomes are 4,147,089 and 4,191,074 bp with GC contents of 28.7% and 28.8%, respectively. Although both strains belong to the same species, whole-genome sequence-based analyses reveal that the strains are phylogenetically distinct. ...[more]

PMID: 33060280

Similar Datasets

Draft Genome Sequences of Two Clostridium algidicarnis Strains Isolated from Meat Juice Samples of Chilled Vacuum-Packed Lamb Meat.

Project description:Clostridium algidicarnis causes blown-pack spoilage of vacuum-packed meat. Here, we report the draft genome sequences of C. algidicarnis strains CM002 and CM003, isolated from unspoiled chilled vacuum-packed lamb. The genome sequences of CM002 and CM003 comprise 2,950,326 and 2,870,995?bp, respectively, and each have a GC content of 30.1%.

| S-EPMC7645660 | biostudies-literature

Draft Genome Sequence of Clostridium estertheticum CEST001, Belonging to a Novel Subspecies of C. estertheticum, Isolated from Chilled Vacuum-Packed Lamb Meat Imported to Switzerland.

Project description:We present the draft genome sequence of Clostridium estertheticum strain CEST001. The genome is 4.8 Mbp long with a GC content of 30.6%. The digital DNA-DNA hybridization values against four C. estertheticum strains indicate that C. estertheticum CEST001 belongs to a novel subspecies of C. estertheticum.

| S-EPMC7427198 | biostudies-literature

Comparative Genome Analysis and Phenotypic Characterization of <i>Clostridium gasigenes</i> CGAS001 Isolated From Chilled Vacuum-Packed Lamb Meat.

Project description:Genomic data for psychrophilic bacteria causing blown pack spoilage (BPS) are limited. This study characterizes the genome of a novel Clostridium gasigenes strain CGAS001 isolated from meat juice sample (MJS) of vacuum-packed lamb meat by comparing it with the type strain C. gasigenes DSM 12272 and five strains representing four other BPS-causing Clostridium sensu stricto species. Phenotypic characteristics of the strain, which include biochemical characteristics, antimicrobial resistance and production of putative polyketide, have been determined. The size of its draft genome is 4.1 Mb with 3,845 coding sequences, 28.7% GC content and 95 RNA genes that include 75 tRNAs, 17 rRNAs, and 3 ncRNAs. Average Nucleotide Identity (ANI) and digital DNA-DNA Hybridization (dDDH) predict that C. gasigenes CGAS001 and DSM 12272 constitute a single species (ANI and dDDH = 98.3% for speciation) but two distinct subspecies (dDDH = 73.3% for subspeciation). The genome is characterized by saccharolytic, lipolytic and proteolytic genes as well as hemolysins and phospholipases, which are consistent with its phenotype. The genome also reveals the ability of C. gasigenes to synthesize polyketides which is demonstrated by the antimicrobial activity of a crude polyketide extract against Listeria monocytogenes and Enterococcus devriesei. The strain is resistant to polymyxin B and streptomycin. The genetic and phenotypic analyses suggest that CGAS001 constitutes a novel subspecies of C. gasigenes adapted to a saprophytic lifestyle and can synthesize narrow spectrum antimicrobial compounds.

| S-EPMC7476324 | biostudies-literature

Project description:One hundred three isolates of Carnobacterium spp. from raw meat were analyzed by random amplification of polymorphic DNA (RAPD) and PCR and were identified by 16S rRNA gene sequencing. Forty-five strains of Carnobacterium maltaromaticum were characterized for their growth capabilities at different temperatures, NaCl concentrations, and pH values and for in vitro lipolytic and proteolytic activities. Moreover, their spoilage potential in meat was investigated by analyzing the release of volatile organic compounds (VOCs) in meat stored in air or vacuum packs. Almost all the strains were able to grow at 4, 10, and 20°C, at pH values of 6 to 9, and in the presence of 2.5% NaCl. The release of VOCs by each strain in beef stored at 4°C in air and vacuum packs was evaluated by headspace solid-phase microextraction (HS-SPME)-gas chromatography-mass spectrometry (GC-MS) analysis. All the meat samples inoculated and stored in air showed higher numbers of VOCs than the vacuum-packed meat samples. Acetoin, 1-octen-3-ol, and butanoic acid were the compounds most frequently found under both storage conditions. The contaminated meat samples were evaluated by a sensory panel; the results indicated that for all sensory odors, no effect of strain was significant (P > 0.05). The storage conditions significantly affected (P < 0.05) the perception of dairy, spoiled-meat, and mozzarella cheese odors, which were more intense in meat stored in air than in vacuum packs but were never very intense. In conclusion, different strains of C. maltaromaticum can grow efficiently in meat stored at low temperatures both in air and in vacuum packs, producing volatile molecules with low sensory impacts, with a negligible contribution to meat spoilage overall.

| S-EPMC3194841 | biostudies-literature

Draft Genome Sequences for Dual-Toxin-Producing Clostridium botulinum Strains.

Project description:Here, we present draft genome sequences for three Clostridium botulinum strains that produce multiple botulinum toxin serotypes. Strains that produce two toxins are rare; however, one of these strains produces subtype B5 and F2 toxins, and two of the strains produce subtype A4 and B5 toxins.

| S-EPMC6346179 | biostudies-literature

Dynamics of Bacterial Communities of Lamb Meat Packaged in Air and Vacuum Pouch during Chilled Storage.

Project description:In this study, the changes in microbial communities of lamb meat packaged in the air (plastic tray, PT) and in a vacuum pouch (VAC) were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) during the storage at 4°C. For the PT lamb, the total viable count (TVC) was 107 CFU/g on Day 5, and the dominated bacteria were Pseudomonas fragi, P. fluorescens, and Acinetobacter spp. For the VAC lamb, the TVC was 107 CFU/g on Day 9, and the dominated bacteria were lactic acid bacteria, including Carnobacterium divergens, C. maltaromaticum, and Lactococcus piscium. One strain of Pseudomonas spp. also appeared in VAC lamb. The relative abundance of Enterobacteriaceae in VAC lamb was higher than that PT lamb, indicating a more important role of Enterobacteriaceae in spoilage for VAC lamb than that of PT lamb. The microbial compositions changed faster in the lamb stored in a PT than that stored in a VAC, and microbial community compositions of the late storage period were largely different from those of the early storage period for both the conditions. The findings of this study may guide improve the lamb hygiene and prolong the shelf life of the lamb.

| S-EPMC6533395 | biostudies-literature

Draft Genome Sequences of Five Brazilian Clostridium botulinum Group III Type D/C Strains.

Project description:Animal botulism is mainly associated with Clostridium botulinum group III-producing neurotoxin types C, C/D, D, and D/C. In this report, we present the draft genome sequences of the first five strains of Clostridium botulinum type D/C isolated in Brazil and used for vaccination purposes.

| S-EPMC5442381 | biostudies-literature

Draft Genome Sequences of 12 Clostridium tyrobutyricum Strains Isolated from Raw Milk and Cheese.

Project description:Clostridium tyrobutyricum is recognized as the main causative agent of late blowing defect-severe spoilage of hard and semihard cheeses. In this work, we present the draft genome sequences of 12 C. tyrobutyricum strains isolated from raw milk and cheese.

| S-EPMC8483699 | biostudies-literature

Draft Genome Sequences of Two Clostridium botulinum Group II Strains Carrying Phage-Like Plasmids.

Project description:Clostridium botulinum is responsible for botulism, a potentially lethal foodborne intoxication. Here, we report the draft genome sequences of C. botulinum group II strains 202F (serotype F) and Hazen (serotype E). The genomes share many similarities, including multiple mobile genetic elements.

| S-EPMC9202375 | biostudies-literature

Draft Genome Sequences of Four Antibiotic-Resistant Salmonella Strains Isolated from Australian Red Meat Animal Species.

Project description:The genome sequences of four antibiotic-resistant Salmonella strains isolated from red meat animals in Australia are presented. Multidrug-resistant Salmonella enterica serovar Heidelberg 329 and Salmonella enterica serovar Typhimurium 2470 harbored an IncHI2 plasmid similar to the multidrug-resistant S. Heidelberg strain N13-01290 plasmid pN13-01290_23 previously isolated in Canada.

| S-EPMC6715880 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data