Ontology highlight
ABSTRACT:
SUBMITTER: Koyama-Honda I
PROVIDER: S-EPMC7563750 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Koyama-Honda Ikuko I Fujiwara Takahiro K TK Kasai Rinshi S RS Suzuki Kenichi G N KGN Kajikawa Eriko E Tsuboi Hisae H Tsunoyama Taka A TA Kusumi Akihiro A
The Journal of cell biology 20201201 12
Using single-molecule imaging with enhanced time resolutions down to 5 ms, we found that CD59 cluster rafts and GM1 cluster rafts were stably induced in the outer leaflet of the plasma membrane (PM), which triggered the activation of Lyn, H-Ras, and ERK and continually recruited Lyn and H-Ras right beneath them in the inner leaflet with dwell lifetimes <0.1 s. The detection was possible due to the enhanced time resolutions employed here. The recruitment depended on the PM cholesterol and saturat ...[more]