Glycosylation of anthocyanins enhances the apoptosis of colon cancer cells by handicapping energy metabolism.

ABSTRACT:

Background

While anthocyanins are proven to be effective in inhibiting tumour cell proliferation, the underlying mechanisms remain unclear. This research aims to explore the glycosylation of anthocyanins in the tumour inhibitory effects and the potential mechanism.

Methods

The tumour inhibitory effect on mouse colon cancer cells (MC38) was examined by MTT and flow cytometric analyses. The inhibitory pathway of anthocyanin was explored by assessment of tumour cell mitochondrial membrane potential (MMP), the caspase-3 and caspase-9 activity, as well as the cell energy metabolism in terms of the glucose uptake, the NAD⁺/NADH ratio and the ATP level.

Results

We found that 500 μM bilberry anthocyanins extract (BAE) induced 48.1% mitochondrial damage, activated t

SUBMITTER: Jing N 

PROVIDER: S-EPMC7566133 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature