High-throughput Minitaturized RNA-Seq Library Preparation.
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ABSTRACT:
SUBMITTER: Mildrum S
PROVIDER: S-EPMC7566612 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
High-throughput Minitaturized RNA-Seq Library Preparation.
Mildrum Samuel S Hendricks Austin A Stortchevoi Alexei A Kamelamela Noelani N Butty Vincent L VL Levine Stuart S SS
Journal of biomolecular techniques : JBT 20201201 4
Advances in next-generation sequencing technologies have allowed RNA sequencing to become an increasingly time efficient, cost-effective, and accessible tool for genomic research. We present here an automated and miniaturized workflow for RNA library preparation that minimizes reagent usage and processing time required per sample to generate Illumina compatible libraries for sequencing. The reduced-volume libraries show similar behavior to full-scale libraries with comparable numbers of genes de ...[more]
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