Isolation, characterisation and detection of breath-derived extracellular vesicles.
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ABSTRACT:
SUBMITTER: Dobhal G
PROVIDER: S-EPMC7566616 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
Isolation, characterisation and detection of breath-derived extracellular vesicles.
Dobhal Garima G Datta Amrita A Ayupova Deanna D Teesdale-Spittle Paul P Goreham Renee V RV
Scientific reports 20201015 1
The physical characterisation, capture and detection of extracellular vesicles (EVs) and exosomes derived from breath condensate is reported. Breath-derived EVs were isolated from breath condensate and captured on a gold substrate using two complimentary methods. The characterised and isolated EVs were detected using surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS). EIS was done using aptamers as a targeting moiety and showed a larger change in resistance between ...[more]
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