Genotypic and Phenotypic Characterization of bla NDM-7-Harboring IncX3 Plasmid in a ST11 Klebsiella pneumoniae Isolated From a Pediatric Patient in China.

ABSTRACT: NDM-7, a variant of New Delhi metallo-beta-lactamases (NDM), has the highest carbapenem-hydrolyzing activity. NDM-7-producing enterobacteria have been reported in many countries. In this study, we reported NDM-7 production in ST11 Klebsiella pneumoniae isolated from a boy hospitalized in the pediatric intensive care unit of a teaching hospital in China. The isolate exhibited resistance to ?-lactam antimicrobials, quinolones, and trimethoprim/sulfamethoxazole, and it harbored bla NDM- 7, bla CTX-M- 15, qnrA, qnrB, and qnrS. The serotype of the isolated K. pneumoniae was assigned as K1, and it contained three virulence genes, including kfuBC, uge, and fim. The bla NDM- 7 gene was located on a conjugative IncX3 plasmid designated as pB14NDM-7. This plasmid was fully sequenced and compared with the available bla NDM- 7-harboring IncX3 plasmids. pB14NDM-7 contained a conserved genetic context of ISkox3-umuD-IS26-?Tn125-IS5-?Tn125-IS3000-?Tn2. pB14NDM-7 showed 99% nucleotide identity and the same genetic context with three bla NDM- 7-harboring IncX3 plasmids obtained from Escherichia coli in China. Our results indicate that IncX3 plasmid may contribute to the prevalence of bla NDM- 7 in China. The high prevalence of NDM variants worldwide highlights the critical need for careful monitoring and control of the rapid dissemination of bla NDM.

SUBMITTER: Shao C 

PROVIDER: S-EPMC7566911 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature