Dataset Information

Non-invasive imaging of radiocesium dynamics in a living animal using a positron-emitting ¹²⁷Cs tracer.

ABSTRACT: Visualizing the dynamics of cesium (Cs) is desirable to understand the impact of radiocesium when accidentally ingested or inhaled by humans. However, visualization of radiocesium in vivo is currently limited to plants. Herein, we describe a method for the production and purification of ¹²⁷Cs and its use in visualizing Cs dynamics in a living animal. The positron-emitting nuclide ¹²⁷Cs was produced using the ¹²⁷I (?, 4n) ¹²⁷Cs reaction, which was induced by irradiation of sodium iodide with a ⁴He²⁺ beam from a cyclotron. We excluded sodium ions by using a material that specifically adsorbs Cs as a purification column and successfully eluted ¹²⁷Cs by flowing a solution of ammonium sulfate into the column. We injected the purified ¹²⁷Cs tracer solution into living rats and the dynamics of Cs were visualized using positron emission tomography; the distributional images showed the same tendency as the results of previous studies using disruptive methods. Thus, this method is useful for the non-invasive investigation of radiocesium in a living animal.

SUBMITTER: Suzui N

PROVIDER: S-EPMC7567787 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Publications

Non-invasive imaging of radiocesium dynamics in a living animal using a positron-emitting 127Cs tracer.

Suzui Nobuo N Shibata Takuya T Yin Yong-Gen YG Funaki Yoshihito Y Kurita Keisuke K Hoshina Hiroyuki H Yamaguchi Mitsutaka M Fujimaki Shu S Seko Noriaki N Watabe Hiroshi H Kawachi Naoki N

Scientific reports 20201015 1

Visualizing the dynamics of cesium (Cs) is desirable to understand the impact of radiocesium when accidentally ingested or inhaled by humans. However, visualization of radiocesium in vivo is currently limited to plants. Herein, we describe a method for the production and purification of 127Cs and its use in visualizing Cs dynamics in a living animal. The positron-emitting nuclide 127Cs was produced using the 127I (α, 4n) 127Cs reaction, which was induc ...[more]

PMID: 33060609

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Project description:BackgroundRice is a major source of dietary intake of cadmium (Cd) for populations that consume rice as a staple food. Understanding how Cd is transported into grains through the whole plant body is necessary for reducing rice Cd concentrations to the lowest levels possible, to reduce the associated health risks. In this study, we have visualized and quantitatively analysed the real-time Cd dynamics from roots to grains in typical rice cultivars that differed in grain Cd concentrations. We used positron-emitting 107Cd tracer and an innovative imaging technique, the positron-emitting tracer imaging system (PETIS). In particular, a new method for direct and real-time visualization of the Cd uptake by the roots in the culture was first realized in this work.ResultsImaging and quantitative analyses revealed the different patterns in time-varying curves of Cd amounts in the roots of rice cultivars tested. Three low-Cd accumulating cultivars (japonica type) showed rapid saturation curves, whereas three high-Cd accumulating cultivars (indica type) were characterized by curves with a peak within 30 min after 107Cd supplementation, and a subsequent steep decrease resulting in maintenance of lower Cd concentrations in their roots. This difference in Cd dynamics may be attributable to OsHMA3 transporter protein, which was recently shown to be involved in Cd storage in root vacuoles and not functional in the high-Cd accumulating cultivars. Moreover, the PETIS analyses revealed that the high-Cd accumulating cultivars were characterized by rapid and abundant Cd transfer to the shoots from the roots, a faster transport velocity of Cd to the panicles, and Cd accumulation at high levels in their panicles, passing through the nodal portions of the stems where the highest Cd intensities were observed.ConclusionsThis is the first successful visualization and quantification of the differences in whole-body Cd transport from the roots to the grains of intact plants within rice cultivars that differ in grain Cd concentrations, by using PETIS, a real-time imaging method.

Dataset Information

Non-invasive imaging of radiocesium dynamics in a living animal using a positron-emitting ¹²⁷Cs tracer.

Publications

Non-invasive imaging of radiocesium dynamics in a living animal using a positron-emitting <sup>127</sup>Cs tracer.

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