Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage.

ABSTRACT: New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques. However, progress in writing DNA is stifled by the continued reliance on chemical synthesis methods. The enzymatic synthesis of DNA is a promising alternative, but thus far has not been well demonstrated in a parallelized manner. Here, we report a multiplexed enzymatic DNA synthesis method using maskless photolithography. Rapid uncaging of Co²⁺ ions by patterned UV light activates Terminal deoxynucleotidyl Transferase (TdT) for spatially-selective synthesis on an array surface. Spontaneous quenching of reactions by the diffusion of excess caging molecules confin

SUBMITTER: Lee H 

PROVIDER: S-EPMC7567835 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature