Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2.
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ABSTRACT:
SUBMITTER: Huang X
PROVIDER: S-EPMC7567891 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2.
Huang Xue X Sun Wei W Cheng Zhi Z Chen Minxuan M Li Xueyan X Wang Jiuyu J Sheng Gang G Gong Weimin W Wang Yanli Y
Nature communications 20201016 1
To understand how the RuvC catalytic domain of Class 2 Cas proteins cleaves DNA, it will be necessary to elucidate the structures of RuvC-containing Cas complexes in their catalytically competent states. Cas12i2 is a Class 2 type V-I CRISPR-Cas endonuclease that cleaves target dsDNA by an unknown mechanism. Here, we report structures of Cas12i2-crRNA-DNA complexes and a Cas12i2-crRNA complex. We reveal the mechanism of DNA recognition and cleavage by Cas12i2, and activation of the RuvC catalytic ...[more]
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