Unknown

Dataset Information

0

Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages.


ABSTRACT: Macrophages play an essential role in the early immune response against Toxoplasma and are the cell type preferentially infected by the parasite in vivo. Interferon gamma (IFNγ) elicits a variety of anti-Toxoplasma activities in macrophages. Using a genome-wide CRISPR screen we identify 353 Toxoplasma genes that determine parasite fitness in naїve or IFNγ-activated murine macrophages, seven of which are further confirmed. We show that one of these genes encodes dense granule protein GRA45, which has a chaperone-like domain, is critical for correct localization of GRAs into the PVM and secretion of GRA effectors into the host cytoplasm. Parasites lacking GRA45 are more susceptible to IFNγ-mediated growth inhibition and have reduced virulence in mice. Together, we identify and characterize an important chaperone-like GRA in Toxoplasma and provide a resource for the community to further explore the function of Toxoplasma genes that determine fitness in IFNγ-activated macrophages.

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7567896 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC10128063 | biostudies-literature
| S-EPMC2931343 | biostudies-literature
| S-EPMC3162014 | biostudies-literature
2020-01-16 | GSE132237 | GEO
| S-EPMC9762571 | biostudies-literature
| S-EPMC8595413 | biostudies-literature
| S-EPMC1140536 | biostudies-literature
| S-EPMC3351351 | biostudies-literature
| S-EPMC4062421 | biostudies-literature
| S-EPMC3695992 | biostudies-literature