Reliable and highly sensitive biosensor from suspended MoS₂ atomic layer on nano-gap electrodes.

ABSTRACT: The uneven morphology and the trapped charges at the surface of the traditionally used supporting substrate-based 2D biosensors produces a scattering effect, which leads to a irregular signals from individually fabricated devices. Though suspended 2D channel material has the potential to overcome scattering effects from the substrates but achieving reliability and selectivity, have been limiting the using of this biosensor technology. Here, we have demonstrated nanogap electrodes fabrication by using the self-assembly technique, which provides suspension to the 2D-MoS₂. These nano-spacing electrodes not only give suspension but also provide robustness strength to the atomic layer, which remains freestanding after coating of the Hafnium oxide (HfO₂) as well as linkers and antibodies. For evaluating the electrical characteristics of suspended MoS₂ FET, gating potential was applied through an electrolyte on the suspended MoS₂ transistor. This helped in achieved a lower subthreshold swing 70 mV/dec and ON/OFF ratio 10⁷. Later, pH detection was conducted at room temperature, which showed an impressive sensitivity of ~880 by changing 1 unit of pH. We have also successfully shown Escherichia coli (E. coli) bacteria sensing from the suspended MoS₂ transistor by functionalizing dielectric layer with E. coli antibodies. The reported biosensor has shown the ~9% of conductance changes with a lower concentration of E. coli (10 CFU/mL; colony-forming unit per mL) as well as maintain the constant sensitivity in three fabricated devices. The obtained enhancement in the sensitivity of devices and its effect on biomolecules detection can be extened to other biomolecules and this type of architecture has the potential to detect COVID-19 viruses based biomolecules.

SUBMITTER: Masurkar N 

PROVIDER: S-EPMC7568125 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature