Dataset Information

Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION.

ABSTRACT: Plastic debris in the ocean form a new ecosystem, termed 'plastisphere', which hosts a variety of marine organisms. Recent studies implemented DNA metabarcoding to characterize the taxonomic composition of the plastisphere in different areas of the world. In this study, we used a modified metabarcoding approach which was based on longer barcode sequences for the characterization of the plastisphere biota. We compared the microbiome of polyethylene food bags after 1 month at sea to the free-living biome in two proximal but environmentally different locations on the Mediterranean coast of Israel. We targeted the full 1.5 kb-long 16S rRNA gene for bacteria and 0.4-0.8 kb-long regions within the 18S rRNA, ITS, tufA and COI loci for eukaryotes. The taxonomic barcodes were sequenced using Oxford Nanopore Technology with multiplexing on a single MinION flow cell. We identified between 1249 and 2141 species in each of the plastic samples, of which 61 species (34 bacteria and 27 eukaryotes) were categorized as plastic-specific, including species that belong to known hydrocarbon-degrading genera. In addition to a large prokaryotes repertoire, our results, supported by scanning electron microscopy, depict a surprisingly high biodiversity of eukaryotes within the plastisphere with a dominant presence of diatoms as well as other protists, algae and fungi.

SUBMITTER: Davidov K

PROVIDER: S-EPMC7568539 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION.

Davidov Keren K Iankelevich-Kounio Evgenia E Yakovenko Iryna I Koucherov Yuri Y Rubin-Blum Maxim M Oren Matan M

Scientific reports 20201016 1

Plastic debris in the ocean form a new ecosystem, termed 'plastisphere', which hosts a variety of marine organisms. Recent studies implemented DNA metabarcoding to characterize the taxonomic composition of the plastisphere in different areas of the world. In this study, we used a modified metabarcoding approach which was based on longer barcode sequences for the characterization of the plastisphere biota. We compared the microbiome of polyethylene food bags after 1 month at sea to the free-livin ...[more]

PMID: 33067509

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Dataset Information

Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION.

Publications

Identification of plastic-associated species in the Mediterranean Sea using DNA metabarcoding with Nanopore MinION.

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