Cryopreservation impairs 3-D migration and cytotoxicity of natural killer cells.
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ABSTRACT:
SUBMITTER: Mark C
PROVIDER: S-EPMC7568558 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
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Cryopreservation impairs 3-D migration and cytotoxicity of natural killer cells.
Mark Christoph C Czerwinski Tina T Roessner Susanne S Mainka Astrid A Hörsch Franziska F Heublein Lucas L Winterl Alexander A Sanokowski Sebastian S Richter Sebastian S Bauer Nina N Angelini Thomas E TE Schuler Gerold G Fabry Ben B Voskens Caroline J CJ
Nature communications 20201016 1
Natural killer (NK) cells are important effector cells in the immune response to cancer. Clinical trials on adoptively transferred NK cells in patients with solid tumors, however, have thus far been unsuccessful. As NK cells need to pass stringent safety evaluation tests before clinical use, the cells are cryopreserved to bridge the necessary evaluation time. Standard degranulation and chromium release cytotoxicity assays confirm the ability of cryopreserved NK cells to kill target cells. Here, ...[more]
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