Dataset Information

Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh.

ABSTRACT: An analysis was accomplished to get information regarding presence of highly toxic and carcinogenic aflatoxin M1 (AFM1) in raw and processed samples of milk applying ELISA (enzyme linked immunosorbent assay). Investigation of a set of 100 samples (n=100) taken from different regional small-scale farms as well as grocery stores of Bangladesh containing three groups of milk including raw (n=50), pasteurized (n=25) and UHT (n=25), exhibited in total 53% AFM1 contamination where 70% contamination was found in raw milk ranging from 22.79-1489.28 ng/kg (mean value 699.07 ng/kg), 52% in pasteurized milk ranging from 18.11-672.18 ng/kg (mean value 99.77 ng/kg) and 20% in UHT milk ranging from 25.07-48.95 ng/kg (mean value 35.46 ng/kg). Among all the positive samples, 75% contaminated samples were above the European Communities prescribed limits (50 ng/kg) while having 25% samples still below this limit whereas 43% samples crossed the accepted limit of US regulations/Codex Alimentarius Commission regulations (500 ng/kg). Thus the findings of this study may lead to increase awareness regarding health impact of aflatoxin M1 and implementation of strict regulations by law enforcement bureaus of Bangladesh.

SUBMITTER: Tarannum N

PROVIDER: S-EPMC7569182 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh.

Tarannum Nourin N Nipa Meher Nigad MN Das Suvra S Parveen Sahana S

Toxicology reports 20201004

An analysis was accomplished to get information regarding presence of highly toxic and carcinogenic aflatoxin M1 (AFM1) in raw and processed samples of milk applying ELISA (enzyme linked immunosorbent assay). Investigation of a set of 100 samples (n=100) taken from different regional small-scale farms as well as grocery stores of Bangladesh containing three groups of milk including raw (n=50), pasteurized (n=25) and UHT (n=25), exhibited in total 53% AFM1 contamination where 70% contamination wa ...[more]

PMID: 33102136

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Project description:Milk is a highly nutritious and perfect natural food for humans. However, when lactating animals feed on Aflatoxin B1 (AFB1)-containing feed, the hydroxyl metabolite aflatoxin M1 (AFM1) contaminates the milk and dairy products. The objective of the current study was to assess the level of AFM1 in raw milk, normally pasteurized milk and Ethiopian cottage cheese collected from value chain actors (producers, collectors, processors and retailers). Cross-sectional study and simple random techniques were used to collect primary samples. A total of 160 composite samples was collected; raw milk (n = 64), pasteurized milk (n = 64) and cheese (n = 32) was analyzed. Quantitative analysis of AFM1 was conducted using enzyme-linked immunosorbent assay (ELISA). The results indicate that AFM1 was detected in all milk products. Results along value chains show that the concentration of AFM1 in raw milk from collectors was significantly higher than from producers, and in pasteurized milk from processors and retailers (p < 0.05). However, no significant (p > 0.05) difference was observed in cottage cheese value-chain actors in all regions. Comparison of AFM1 mean values among all dairy products shows that raw milk had a significantly higher concentration of AFM1 followed by pasteurized milk and cottage cheese. However, there was no significant difference between raw and pasteurized milk (p > 0.05). The mean AFM1 contamination in milk products ranged from 0.137 to 0.319 µg/L (mean value 0.285 µg/L). The contamination percentages of AFM1 in raw milk (62.50%), pasteurized milk (67.20%) and cottage cheese (25%) were above the regulatory limit set by the European Union (EU) (0.05 µg/L). According to USA/Ethiopian Standard (US/ES) (0.50 µg/L), 21.87%, 25% and 1% exceeded the regulatory limit for the above products, respectively. The overall prevalence (56.88%) was above the EU regulatory limit and 19.38% over US/ES regulations. Therefore, to provide accurate information about the health risk to consumers, there is a need to conduct risk assessment studies in consumers of milk and dairy products at different age groups.

Dataset Information

Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh.

Publications

Aflatoxin M1 detection by ELISA in raw and processed milk in Bangladesh.

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