High-throughput analysis of adaptation using barcoded strains of Saccharomyces cerevisiae.

ABSTRACT:

Background

Experimental evolution of microbes can be used to empirically address a wide range of questions about evolution and is increasingly employed to study complex phenomena ranging from genetic evolution to evolutionary rescue. Regardless of experimental aims, fitness assays are a central component of this type of research, and low-throughput often limits the scope and complexity of experimental evolution studies. We created an experimental evolution system in Saccharomyces cerevisiae that utilizes genetic barcoding to overcome this challenge.

Results

We first confirm that barcode insertions do not alter fitness and that barcode sequencing can be used to efficiently detect fitness differences via pooled competition-based fitness assays. Next, we examine the effe

SUBMITTER: Fasanello VJ 

PROVIDER: S-EPMC7571412 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature