Detection of SARS-CoV-2 RNA by multiplex RT-qPCR.
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ABSTRACT:
SUBMITTER: Kudo E
PROVIDER: S-EPMC7571696 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
Detection of SARS-CoV-2 RNA by multiplex RT-qPCR.
Kudo Eriko E Israelow Benjamin B Vogels Chantal B F CBF Lu Peiwen P Wyllie Anne L AL Tokuyama Maria M Venkataraman Arvind A Brackney Doug E DE Ott Isabel M IM Petrone Mary E ME Earnest Rebecca R Lapidus Sarah S Muenker M Catherine MC Moore Adam J AJ Casanovas-Massana Arnau A Omer Saad B SB Dela Cruz Charles S CS Farhadian Shelli F SF Ko Albert I AI Grubaugh Nathan D ND Iwasaki Akiko A
PLoS biology 20201007 10
The current quantitative reverse transcription PCR (RT-qPCR) assay recommended for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing in the United States requires analysis of 3 genomic targets per sample: 2 viral and 1 host. To simplify testing and reduce the volume of required reagents, we devised a multiplex RT-qPCR assay to detect SARS-CoV-2 in a single reaction. We used existing N1, N2, and RP primer and probe sets by the Centers for Disease Control and Prevention, but sub ...[more]
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