Visualization of mechanical stress-mediated Ca²⁺ signaling in the gut using intravital imaging.

ABSTRACT: Mechanosensory systems have been implicated in the maintenance of gut homeostasis, but details on the related mechanisms are scarce. Recently, we generated a conditional Ca²⁺ biosensor yellow cameleon 3.60 (YC3.60)-expressing transgenic mouse model and established a five-dimensional (5D; x, y, z, time, and Ca²⁺) intravital imaging system for investigating lymphoid tissues and enteric epithelial cell responses. To validate this gut-sensing system, we visualized responses of enteric nervous system (ENS) cells in Nestin-Cre/YC3.60^flox mice with specific YC3.60 expression. The ENS, including the myenteric (Auerbach's) and submucous (Meissner's) plexuses, could be visualized without staining in this mouse line, indicating that the probe produced sufficient fluor

SUBMITTER: Aihara Y 

PROVIDER: S-EPMC7573108 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature