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Dstac Regulates Excitation-Contraction Coupling in Drosophila Body Wall Muscles.


ABSTRACT: Stac3 regulates excitation-contraction coupling (EC coupling) in vertebrate skeletal muscles by regulating the L-type voltage-gated calcium channel (Cav channel). Recently a stac-like gene, Dstac, was identified in Drosophila and found to be expressed by both a subset of neurons and muscles. Here, we show that Dstac and Dmca1D, the Drosophila L-type Cav channel, are necessary for normal locomotion by larvae. Immunolabeling with specific antibodies against Dstac and Dmca1D found that Dstac and Dmca1D are expressed by larval body-wall muscles. Furthermore, Ca2+ imaging of muscles of Dstac and Dmca1D deficient larvae found that Dstac and Dmca1D are required for excitation-contraction coupling. Finally, Dstac appears to be required for normal expression levels of Dmca1D in body-wall muscles. These results suggest that Dstac regulates Dmca1D during EC coupling and thus muscle contraction.

SUBMITTER: Hsu IU 

PROVIDER: S-EPMC7573238 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Dstac Regulates Excitation-Contraction Coupling in <i>Drosophila</i> Body Wall Muscles.

Hsu I-Uen IU   Linsley Jeremy W JW   Reid Lilly E LE   Hume Richard I RI   Leflein Ari A   Kuwada John Y JY  

Frontiers in physiology 20201006


Stac3 regulates excitation-contraction coupling (EC coupling) in vertebrate skeletal muscles by regulating the L-type voltage-gated calcium channel (Ca<sub>v</sub> channel). Recently a <i>stac</i>-like gene, <i>Dstac</i>, was identified in <i>Drosophila</i> and found to be expressed by both a subset of neurons and muscles. Here, we show that Dstac and Dmca1D, the <i>Drosophila</i> L-type Ca<sub>v</sub> channel, are necessary for normal locomotion by larvae. Immunolabeling with specific antibodie  ...[more]

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