High throughput single-cell detection of multiplex CRISPR-edited gene modifications.
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ABSTRACT:
SUBMITTER: Ten Hacken E
PROVIDER: S-EPMC7574538 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
High throughput single-cell detection of multiplex CRISPR-edited gene modifications.
Ten Hacken Elisa E Clement Kendell K Li Shuqiang S Hernández-Sánchez María M Redd Robert R Wang Shu S Ruff David D Gruber Michaela M Baranowski Kaitlyn K Jacob Jose J Flynn James J Jones Keith W KW Neuberg Donna D Livak Kenneth J KJ Pinello Luca L Wu Catherine J CJ
Genome biology 20201020 1
CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence o ...[more]
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