An insight into anti-adipogenic properties of an Oroxylum indicum (L.) Kurz extract.

ABSTRACT: BACKGROUND:Oroxylum indicum fruit extract (OIE) has been reported to inhibit the development of adipocytes. However, the exact mechanism of its metabolic activity is not clearly defined. This study attempted to investigate whether OIE was involved in disrupting the cell cycle, glucose metabolism, and mitochondrial function in 3?T3-L1 cells. METHODS:The effect of the OIE on cell cycle progression was measured by flow cytometry along with observing the expression of the cycle regulator by immunoblotting. The effect of the OIE on glucose metabolism was investigated. The amount of glucose uptake (2-NBDG) influenced by insulin was determined as well as the protein tyrosine phosphorylation (PY20), and glucose transporter4 (GLUT4) expression was determined by immunoblotting assay. Mitochondria are also essential to metabolic processes. This study investigated mitochondrial activity using fluorescent lipophilic carbocyanine dye (JC-1) and mitochondria mass by MitoTracker Green (MTG) staining fluorescence dyes. Finally, cellular ATP concentration was measured using an ATP chemiluminescence assay. RESULTS:Treatment with OIE plus adipogenic stimulators for 24?h arrested cell cycle progression in the G2/M phase. Moreover, 200??g/mL of OIE significantly diminished the expression of the insulin receptor (IR) and GLUT4 protein compared to the untreated-adipocytes (P 

SUBMITTER: Hengpratom T 

PROVIDER: S-EPMC7576871 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature