Browse
Submit Data
Databases
API
Help

Dataset Information

28 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Author Correction: Reconstructed Skin Models Revealed Unexpected Differences in Epidermal African and Caucasian Skin.

ABSTRACT: An amendment to this paper has been published and can be accessed via a link at the top of the paper.

SUBMITTER: Girardeau-Hubert S

PROVIDER: S-EPMC7577967 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Author Correction: Reconstructed Skin Models Revealed Unexpected Differences in Epidermal African and Caucasian Skin.

Girardeau-Hubert Sarah S Deneuville Céline C Pageon Hervé H Abed Kahina K Tacheau Charlotte C Cavusoglu Nükhet N Donovan Mark M Bernard Dominique D Asselineau Daniel D

Scientific reports 20201021 1

An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...[more]

PMID: 33087850

Similar Datasets

Reconstructed Skin Models Revealed Unexpected Differences in Epidermal African and Caucasian Skin.

Project description:Clinical observations of both normal and pathological skin have shown that there is a heterogeneity based on the skin origin type. Beside external factors, intrinsic differences in skin cells could be a central element to determine skin types. This study aimed to understand the in vitro behaviour of epidermal cells of African and Caucasian skin types in the context of 3D reconstructed skin. Full-thickness skin models were constructed with site matched human keratinocytes and papillary fibroblasts to investigate potential skin type related differences. We report that reconstructed skin epidermis exhibited remarkable differences regarding stratification and differentiation according to skin types, as demonstrated by histological appearance, gene expression analysed by DNA microarray and quantitative proteomic analysis. Signalling pathways and processes related to terminal differentiation and lipid/ceramide metabolism were up-regulated in epidermis constructed with keratinocytes from Caucasian skin type when compared to that of keratinocytes from African skin type. Specifically, the expression of proteins involved in the processing of filaggrins was found different between skin models. Overall, we show unexpected differences in epidermal morphogenesis and differentiation between keratinocytes of Caucasian and African skin types in in vitro reconstructed skin containing papillary fibroblasts that could explain the differences in ethnic related skin behaviour.

| S-EPMC6520399 | biostudies-literature

Microarray of reconstructed epidermis from skin models made with cells from African and Caucasian Skin type

Project description:The aim of this study was to analyze the in vitro biological epidermal processes occurring in reconstructed skins using cells from breast skin of African and Caucasian skin type color. A exploration of mRNA expression levels in the epidermis of reconstructed skin was undertaken to elucidate the differential in vitro functions of keratinocytes. The reconstruction of skin models was made with keratinocytes and fibroblasts from four different donors per skin type and experiments were conducted in triplicate for each donor. At the end of culture, the epidermis from reconstructed skin was manually separated from the dermal equivalent part in order to analyse gene expression in keratinocytes only. RNA samples were labelled with biotin and hybridation was performed on Affymetrix Human Genome U133 + PM Array Plates.

2020-08-10 | E-MTAB-9433 | biostudies-arrayexpress

Author Correction: Transcriptional landscape of epithelial and immune cell populations revealed through FACS-seq of healthy human skin.

Project description: Not available

| S-EPMC8763905 | biostudies-literature

Utilization of patterned bioprinting for heterogeneous and physiologically representative reconstructed epidermal skin models.

Project description:Organotypic skin tissue models have decades of use for basic research applications, the treatment of burns, and for efficacy/safety evaluation studies. The complex and heterogeneous nature of native human skin however creates difficulties for the construction of physiologically comparable organotypic models. Within the present study, we utilized bioprinting technology for the controlled deposition of separate keratinocyte subpopulations to create a reconstructed epidermis with two distinct halves in a single insert, each comprised of a different keratinocyte sub-population, in order to better model heterogonous skin and reduce inter-sample variability. As an initial proof-of-concept, we created a patterned epidermal skin model using GPF positive and negative keratinocyte subpopulations, both printed into 2 halves of a reconstructed skin insert, demonstrating the feasibility of this approach. We then demonstrated the physiological relevance of this bioprinting technique by generating a heterogeneous model comprised of dual keratinocyte population with either normal or low filaggrin expression. The resultant model exhibited a well-organized epidermal structure with each half possessing the phenotypic characteristics of its constituent cells, indicative of a successful and stable tissue reconstruction. This patterned skin model aims to mimic the edge of lesions as seen in atopic dermatitis or ichthyosis vulgaris, while the use of two populations within a single insert allows for paired statistics in evaluation studies, likely increasing study statistical power and reducing the number of models required per study. This is the first report of human patterned epidermal model using a predefined bioprinted designs, and demonstrates the relevance of bioprinting to faithfully reproduce human skin microanatomy.

| S-EPMC7973417 | biostudies-literature

Author Correction: Skin tolerant inactivation of multiresistant pathogens using far-UVC LEDs.

Project description: Not available

| S-EPMC9095698 | biostudies-literature

Proteomic study of in vitro epidermis of reconstructed skins made with cells from African or Caucasian skin type

Project description:This study aimed to understand the in vitro behaviour of epidermal cells of African and Caucasian skin types in the context of 3D reconstructed skin. Reconstructed skins epidermis made with cells isolated from skin of African or Caucasian skin type exhibited high differences in stratification and differentiation. The objective of this study is a first global approach to identify at the protein level the differences between reconstructed skins.

| MSV000086147 | MassIVE

Differences in Timely Cancer Diagnosis in African American and Caucasian Patients With Newly Diagnosed Colorectal Cancer

Project description:RATIONALE: Gathering information about timely diagnosis in African American and Caucasian patients with newly diagnosed colorectal cancer may help doctors learn more about factors that influence a diagnosis and plan the best treatment. PURPOSE: This phase I trial is studying differences in timely cancer diagnosis in African American patients and in Caucasian patients with newly diagnosed colorectal cancer.

| 2068885 | ecrin-mdr-crc

Author Correction: A Geodetic Strain Rate Model for the East African Rift System.

Project description:An amendment to this paper has been published and can be accessed via a link at the top of the paper.

| S-EPMC6949277 | biostudies-literature

Author Correction: Functional connectome differences in individuals with hallucinations across the psychosis continuum.

Project description: Not available

| S-EPMC8046756 | biostudies-literature

Author Correction: The balance of metagenomic elements shapes the skin microbiome in acne and health.

Project description:An amendment to this paper has been published and can be accessed via a link at the top of the paper.

| S-EPMC7118076 | biostudies-literature