Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru.
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ABSTRACT:
SUBMITTER: Flores JA
PROVIDER: S-EPMC7578116 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
Publications
Detection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru.
Flores Juan A JA Calderón Roger R Mesman Annelies W AW Soto Martin M Coit Julia J Aliaga Juan J Mendoza Milagros M Leon Segundo R SR Konda Kelika K Mestanza Francisco M FM Mendoza Carlos J CJ Lecca Leonid L Murray Megan B MB Holmberg Rebecca C RC Pollock Nira R NR Franke Molly F MF
The Pediatric infectious disease journal 20201101 11
We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%). ...[more]
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