Fractionated small cell-free DNA increases possibility to detect cancer-related gene mutations in advanced colorectal cancer.

ABSTRACT:

Background and aim

Liquid biopsy is a method that can efficiently detect tumor genetic abnormalities from body fluids such as blood and urine. Detection sensitivity and the available number of mutations in cell-free DNA (cfDNA) are limited. In this study, we develop a highly sensitive and comprehensive method to detect mutations from cfDNA by concentrating tumor fractions of small cfDNA in advanced colorectal cancers.

Methods

Biopsied specimens and 37 serum samples were collected from 27 patients with advanced colorectal carcinoma. A serum-extracted cfDNA was divided into enriched fractionated small cfDNA and unfractionated cfDNA. Both cfDNAs were subjected to digital polymerase chain reaction (PCR) to evaluate their KRAS, BRAF, CDKN2A, and TP53 status. Consequ

SUBMITTER: Ishida Y 

PROVIDER: S-EPMC7578331 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature