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Chromatin-Bound Proteome Profiling by Genome Capture.

ABSTRACT: De novo identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and de novo identification of chromatin-bound proteins. Here, we detail a step-by-step protocol to survey the cellular chromatin-bound proteome in a simple, robust, and unbiased manner. For complete details on the use and execution of this protocol, please refer to Aranda et al. (2019).

SUBMITTER: Aranda S

PROVIDER: S-EPMC7580076 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Chromatin-Bound Proteome Profiling by Genome Capture.

Aranda Sergi S Borràs Eva E Sabidó Eduard E Di Croce Luciano L

STAR protocols 20200603 1

<i>De novo</i> identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and <i>de novo</i> identification of ch ...[more]

PMID: 33111072

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