Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome.
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ABSTRACT:
SUBMITTER: Bull C
PROVIDER: S-EPMC7580198 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
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Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome.
Büll Christian C Joshi Hiren J HJ Clausen Henrik H Narimatsu Yoshiki Y
STAR protocols 20200603 1
Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans ...[more]
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