Processing Human Thymic Tissue for Single Cell RNA-Seq.
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ABSTRACT:
SUBMITTER: Le J
PROVIDER: S-EPMC7580228 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Processing Human Thymic Tissue for Single Cell RNA-Seq.
Le Justin J Ha Vi Luan VL Luong Annie A Parekh Chintan C
STAR protocols 20200824 2
Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34<sup>+</sup> progenitor and more differentiated CD34<sup>-</sup> fractions from post-natal thymic tissue to study thymopoiesis. CD34<sup>+</sup> cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched CD34<sup>+</sup> cells. For complete details ...[more]
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