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Processing Human Thymic Tissue for Single Cell RNA-Seq.


ABSTRACT: Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34+ progenitor and more differentiated CD34- fractions from post-natal thymic tissue to study thymopoiesis. CD34+ cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched CD34+ cells. For complete details on the use and execution of this protocol, please refer to Le et al. (2020).

SUBMITTER: Le J 

PROVIDER: S-EPMC7580228 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Processing Human Thymic Tissue for Single Cell RNA-Seq.

Le Justin J   Ha Vi Luan VL   Luong Annie A   Parekh Chintan C  

STAR protocols 20200824 2


Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34<sup>+</sup> progenitor and more differentiated CD34<sup>-</sup> fractions from post-natal thymic tissue to study thymopoiesis. CD34<sup>+</sup> cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched CD34<sup>+</sup> cells. For complete details  ...[more]

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