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Generation of Sequencing Libraries for Structural Analysis of Bacterial 5' UTRs.

ABSTRACT: The structure of 5' untranslated regions (5' UTRs) of bacterial mRNAs often determines the fate of the transcripts. Using a dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) approach, we developed a protocol to generate sequence libraries to determine the base-pairing status of adenines and cytosines in the 5' UTRs of bacterial mRNAs. Our method increases the sequencing depth of the 5' UTRs and allows detection of changes in their structures by sequencing libraries of moderate sizes. For complete details on the use and execution of this protocol, please refer to Ignatov et al. (2020).

SUBMITTER: Ignatov D

PROVIDER: S-EPMC7580233 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Generation of Sequencing Libraries for Structural Analysis of Bacterial 5' UTRs.

Ignatov Dmitriy D Vaitkevicius Karolis K Johansson Jörgen J

STAR protocols 20200606 2

The structure of 5' untranslated regions (5' UTRs) of bacterial mRNAs often determines the fate of the transcripts. Using a dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) approach, we developed a protocol to generate sequence libraries to determine the base-pairing status of adenines and cytosines in the 5' UTRs of bacterial mRNAs. Our method increases the sequencing depth of the 5' UTRs and allows detection of changes in their structures by sequencing libraries of moderate s ...[more]

PMID: 33111092

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