Ontology highlight
ABSTRACT:
SUBMITTER: Wiegand T
PROVIDER: S-EPMC7580607 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
The CRISPR journal 20201001 5
CRISPR-associated proteins 1 and 2 (Cas1-2) are necessary and sufficient for new spacer acquisition in some CRISPR-Cas systems (e.g., type I-E), but adaptation in other systems (e.g., type II-A) involves the crRNA-guided surveillance complex. Here we show that the type I-F Cas1-2/3 proteins are necessary and sufficient to produce low levels of spacer acquisition, but the presence of the type I-F crRNA-guided surveillance complex (Csy) improves the efficiency of adaptation and significantly incre ...[more]