Ontology highlight
ABSTRACT:
SUBMITTER: Woodruff G
PROVIDER: S-EPMC7581002 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Woodruff Grace G Phillips Naomi N Carromeu Cassiano C Guicherit Oivin O White Alistair A Johnson McCay M Zanella Fabian F Anson Blake B Lovenberg Timothy T Bonaventure Pascal P Harrington Anthony W AW
PloS one 20201022 10
Human induced Pluripotent Stem Cells (iPSCs) are a powerful tool to dissect the biology of complex human cell types such as those of the central nervous system (CNS). However, robust, high-throughput platforms for reliably measuring activity in human iPSC-derived neuronal cultures are lacking. Here, we assessed 3D cultures of cortical neurons and astrocytes displaying spontaneous, rhythmic, and highly synchronized neural activity that can be visualized as calcium oscillations on standard high-th ...[more]