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A Survey of Barley PIP Aquaporin Ionic Conductance Reveals Ca2+-Sensitive HvPIP2;8 Na+ and K+ Conductance.


ABSTRACT: Some plasma membrane intrinsic protein (PIP) aquaporins can facilitate ion transport. Here we report that one of the 12 barley PIPs (PIP1 and PIP2) tested, HvPIP2;8, facilitated cation transport when expressed in Xenopus laevis oocytes. HvPIP2;8-associated ion currents were detected with Na+ and K+, but not Cs+, Rb+, or Li+, and was inhibited by Ba2+, Ca2+, and Cd2+ and to a lesser extent Mg2+, which also interacted with Ca2+. Currents were reduced in the presence of K+, Cs+, Rb+, or Li+ relative to Na+ alone. Five HvPIP1 isoforms co-expressed with HvPIP2;8 inhibited the ion conductance relative to HvPIP2;8 alone but HvPIP1;3 and HvPIP1;4 with HvPIP2;8 maintained the ion conductance at a lower level. HvPIP2;8 water permeability was similar to that of a C-terminal phosphorylation mimic mutant HvPIP2;8 S285D, but HvPIP2;8 S285D showed a negative linear correlation between water permeability and ion conductance that was modified by a kinase inhibitor treatment. HvPIP2;8 transcript abundance increased in barley shoot tissues following salt treatments in a salt-tolerant cultivar Haruna-Nijo, but not in salt-sensitive I743. There is potential for HvPIP2;8 to be involved in barley salt-stress responses, and HvPIP2;8 could facilitate both water and Na+/K+ transport activity, depending on the phosphorylation status.

SUBMITTER: Tran STH 

PROVIDER: S-EPMC7582361 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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A Survey of Barley PIP Aquaporin Ionic Conductance Reveals Ca<sup>2+</sup>-Sensitive <i>HvPIP2;8</i> Na<sup>+</sup> and K<sup>+</sup> Conductance.

Tran Sen Thi Huong STH   Horie Tomoaki T   Imran Shahin S   Qiu Jiaen J   McGaughey Samantha S   Byrt Caitlin S CS   Tyerman Stephen D SD   Katsuhara Maki M  

International journal of molecular sciences 20200927 19


Some plasma membrane intrinsic protein (PIP) aquaporins can facilitate ion transport. Here we report that one of the 12 barley PIPs (PIP1 and PIP2) tested, <i>HvPIP2;8</i>, facilitated cation transport when expressed in <i>Xenopus laevis</i> oocytes. <i>HvPIP2;8</i>-associated ion currents were detected with Na<sup>+</sup> and K<sup>+</sup>, but not Cs<sup>+</sup>, Rb<sup>+</sup>, or Li<sup>+</sup>, and was inhibited by Ba<sup>2+</sup>, Ca<sup>2+</sup>, and Cd<sup>2+</sup> and to a lesser extent  ...[more]

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