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Two Alternative Splicing Variants of AtERF73/HRE1, HRE1? and HRE1?, Have Differential Transactivation Activities in Arabidopsis.

ABSTRACT: AtERF73/HRE1 is an AP2/ERF transcription factor in Arabidopsis and has two distinct alternative splicing variants, HRE1? and HRE1?. In this study, we examined the differences between the molecular functions of HRE1? and HRE1?. We found that HRE1? and HRE1? are both involved in hypoxia response and root development and have transactivation activity. Two conserved motifs in the C-terminal region of HRE1? and HRE1?, EELL and LWSY-like, contributed to their transactivation activity, specifically the four E residues in the EELL motif and the MGLWS amino acid sequence at the end of the LWSY-like motif. The N-terminal region of HRE1? also showed transactivation activity, mediated by the VDDG motif, whereas that of HRE1? did not. The transactivation activity of HRE1? was stronger than that of HRE1? in Arabidopsis protoplasts. Both transcription factors transactivated downstream genes via the GCC box. RNA-sequencing analysis further supported that both HRE1? and HRE1? might regulate gene expression associated with the hypoxia stress response, although they may transactivate different subsets of genes in downstream pathways. Our results, together with previous studies, suggested that HRE1? and HRE1? differentially transactivate downstream genes in hypoxia response and root development in Arabidopsis.

SUBMITTER: Seok HY

PROVIDER: S-EPMC7582492 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i>.

Seok Hye-Yeon HY Ha Jimin J Lee Sun-Young SY Bae Hyoungjoon H Moon Yong-Hwan YH

International journal of molecular sciences 20200923 19

AtERF73/HRE1 is an AP2/ERF transcription factor in <i>Arabidopsis</i> and has two distinct alternative splicing variants, HRE1α and HRE1β. In this study, we examined the differences between the molecular functions of HRE1α and HRE1β. We found that HRE1α and HRE1β are both involved in hypoxia response and root development and have transactivation activity. Two conserved motifs in the C-terminal region of HRE1α and HRE1β, EELL and LWSY-like, contributed to their transactivation activity, specifica ...[more]

PMID: 32977426

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Project description:BackgroundCa2+-dependent activator protein 2 (CAPS2/CADPS2) is a secretory vesicle-associated protein involved in the release of neurotrophin. We recently reported that an aberrant, alternatively spliced CAPS2 mRNA that lacks exon 3 (CAPS2Deltaexon3) is detected in some patients with autism. Splicing variations in mouse CAPS2 and their expression and functions remain unclear.ResultsIn this study, we defined 31 exons in the mouse CAPS2 gene and identified six alternative splicing variants, CAPS2a-f. CAPS2a is an isoform lacking exons 22 and 25, which encode part of the Munc13-1-homologous domain (MHD). CAPS2b lacks exon 25. CAPS2c lacks exons 11 and 22. CAPS2d, 2e, and 2f have C-terminal deletions from exon 14, exon 12, and exon 5, respectively. On the other hand, a mouse counterpart of CAPS2Deltaexon3 was not detected in the mouse tissues tested. CAPS2b was expressed exclusively in the brain, and the other isoforms were highly expressed in the brain, but also in some non-neural tissues. In the brain, all isoforms showed predominant expression patterns in the cerebellum. In the developing cerebellum, CAPS2b showed an up-regulated expression pattern, whereas the other isoforms exhibited transiently peaked expression patterns. CAPS2 proteins were mostly recovered in soluble fractions, but some were present in membrane fractions, except for CAPS2c and 2f, both of which lack the PH domain, suggesting that the PH domain is important for membrane association. In contrast to CAPS2a and 2b, CAPS2c showed slightly decreased BDNF-releasing activity, which is likely due to the C-terminal truncation of the PH domain in CAPS2c.ConclusionThis study indicates that, in mouse, there are six splicing variants of CAPS2 (CAPS2a-f), and that these are subdivided into two groups: a long form containing the C-terminal MHD and a short form lacking the C-terminal MHD. These results demonstrate that the splicing variations correlate with their expression patterns and intracellular distribution, and affect BDNF release; however, whether or not the short forms possess activities other than BDNF release, for example as natural dominant-negative isoforms, remains to be determined.

Dataset Information

Two Alternative Splicing Variants of AtERF73/HRE1, HRE1? and HRE1?, Have Differential Transactivation Activities in Arabidopsis.

Publications

Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in <i>Arabidopsis</i>.

Similar Datasets

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