Dataset Information

MiR-224 targets BTRC and promotes cell migration and invasion in colorectal cancer.

ABSTRACT: Our study aims to investigate the impact of miR-224 on cell migration and invasion in colorectal cancer (CRC) as well as its molecular mechanisms. The results showed that miR-224 was significantly upregulated in CRC compared to normal tissues via the TCGA database. Overexpression of miR-224 promoted CRC cell migration and invasion, while inhibition of miR-224 demonstrated the opposite result via transwell assays. In addition, we found that BTRC was a target gene of miR-224 through the miRecords database and dual-luciferase assay, while western blot together with RT-qPCR showed that inhibition of miR-224 led to elevated BTRC expression in protein level but not in mRNA level, and also decreased the expression of ?-catenin. In reference to the Human Protein Atlas, BTRC protein expression was higher in normal tissues than in CRC tissues. In conclusion, miR-224 regulates its target BTRC protein expression and its related Wnt/?-catenin pathway. Its impact on cell migration and invasion in CRC cells suggested that miR-224 could be a prospective therapeutic target for early-stage non-metastatic CRC.

SUBMITTER: Zheng Q

PROVIDER: S-EPMC7585582 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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miR-224 targets <i>BTRC</i> and promotes cell migration and invasion in colorectal cancer.

Zheng Qi Q Yu Jane J JJ Li Chenggang C Li Jiali J Wang Jiping J Wang Shuyang S

3 Biotech 20201024 11

Our study aims to investigate the impact of miR-224 on cell migration and invasion in colorectal cancer (CRC) as well as its molecular mechanisms. The results showed that miR-224 was significantly upregulated in CRC compared to normal tissues via the TCGA database. Overexpression of miR-224 promoted CRC cell migration and invasion, while inhibition of miR-224 demonstrated the opposite result via transwell assays. In addition, we found that <i>BTRC</i> was a target gene of miR-224 through the miR ...[more]

PMID: 33117626

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Dataset Information

MiR-224 targets BTRC and promotes cell migration and invasion in colorectal cancer.

Publications

miR-224 targets <i>BTRC</i> and promotes cell migration and invasion in colorectal cancer.

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