Ontology highlight
ABSTRACT:
SUBMITTER: Cruvinel E
PROVIDER: S-EPMC7586033 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Cruvinel Estela E Ogusuku Isabella I Cerioni Rosanna R Rodrigues Sirlene S Gonçalves Jéssica J Góes Maria Elisa ME Alvim Juliana Morais JM Silva Anderson Carlos AC Lino Vanesca de Souza VS Boccardo Enrique E Goulart Ernesto E Pereira Alexandre A Dariolli Rafael R Valadares Marcos M Biagi Diogo D
SAGE open medicine 20201022
<h4>Objectives</h4>To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency.<h4>Methods</h4>Cells were kept in culture for over 50 passages, following a structured chronogram of passage and monitoring cell growth by population doubling time calculation and cell confluence. Standard procedures for human induced pluripotent stem cel ...[more]