Dataset Information

Measuring beta-galactose exposure on platelets: Standardization and healthy reference values.

ABSTRACT: Background:Correct diagnosis of the cause of thrombocytopenia is crucial for the appropriate management of patients. Hyposialylation/desialylation (characterized by abnormally high ?-galactose exposure) accelerates platelet clearance and can lead to thrombocytopenia. However, the reference range for ?-galactose exposure in healthy individuals has not been defined previously. Objective:The objective of the present study was to develop a standardized assay of platelet ?-galactose exposure for implementation in a clinical laboratory. Methods:?-Galactose exposure was measured in platelet-rich plasma by using flow cytometry and Ricinus communis agglutinin (RCA). A population of 120 healthy adults was recruited to study variability. Results:We determined an optimal RCA concentration of 12.5 ?g/mL. The measure was stable for up to 4 hours (mean fluorescence intensity [MFI]-RCA: 1233 ± 329 at 0 hour and 1480 ± 410 at 4 hours). The platelet count did not induce a variation of RCA and the measure of RCA was stable when tested up to 24 hours after blood collection (MFI-RCA: 1252 ± 434 at day 0 and 1140 ± 297 24 hours after blood sampling). To take into account the platelet size, results should be expressed as RCA/forward scatter ratio. We used the assay to study variability in 120 healthy adults, and we found that the ratio is independent of sex and blood group. Conclusion:We defined a normal range in a healthy population and several preanalytical and analytical variables were evaluated, together with positive and negative controls. This assay may assist in the diagnosis of thrombocytopenic diseases linked to changes in ?-galactose exposure.

SUBMITTER: Lasne D

PROVIDER: S-EPMC7586713 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Measuring beta-galactose exposure on platelets: Standardization and healthy reference values.

Lasne Dominique D Pascreau Tiffany T Darame Sadyo S Bourrienne Marie-Charlotte MC Tournoux Peggy P Philippe Aurélien A Ziachahabi Sara S Suarez Felipe F Marcais Ambroise A Dupont Annabelle A Denis Cécile V CV Kauskot Alexandre A Borgel Delphine D

Research and practice in thrombosis and haemostasis 20200602 5

<h4>Background</h4>Correct diagnosis of the cause of thrombocytopenia is crucial for the appropriate management of patients. Hyposialylation/desialylation (characterized by abnormally high β-galactose exposure) accelerates platelet clearance and can lead to thrombocytopenia. However, the reference range for β-galactose exposure in healthy individuals has not been defined previously.<h4>Objective</h4>The objective of the present study was to develop a standardized assay of platelet β-galactose ex ...[more]

PMID: 33134771

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Project description:IntroductionThe outcomes anxiety and depression are measured frequently by healthcare providers to assess the impact of a disease, but with numerous instruments. PROMIS item banks provide an opportunity for standardized measurement. Cross-cultural validity of measures and the availability of reference values are prerequisites for standardized measurement.MethodsPROMIS Anxiety and Depression item banks were completed by 1002 representative Dutch persons. To evaluate cross-cultural validity, data from US participants in PROMIS wave 1 were used and differential item functioning (DIF) was investigated, using an iterative hybrid of logistic regression and item response theory. McFadden's pseudo R2-change of 2% was the critical threshold. The impact of any DIF on full item banks and short forms was investigated. To obtain Dutch reference values, T-scores for anxiety and depression were calculated for the complete Dutch sample, and age-group and gender subpopulations. Thresholds corresponding to normal limits, mild, moderate and severe symptoms were computed.ResultsIn both item banks, two items had DIF but with minimal impact on population level T-scores for full item banks and short forms. The Dutch general population had a T-score of 49.9 for anxiety and 49.6 for depression, similar to the T-scores of 50.0 of the US general population. T-scores for age-group and gender subpopulations were also similar to T-scores of the US general population. Thresholds for mild, moderate and severe anxiety and depression were set to 55, 60 and 70, identical to US thresholds.ConclusionsThe limited number of items with DIF and its minimal impact, enables the use of standard (US) item parameters and comparisons of scores between Dutch and US populations. The Dutch reference values provide an important tool for healthcare professionals and researchers to evaluate and interpret symptoms of anxiety and depression, stimulating the uptake of PROMIS measures, and contributing to standardized outcome measurement.

Dataset Information

Measuring beta-galactose exposure on platelets: Standardization and healthy reference values.

Publications

Measuring beta-galactose exposure on platelets: Standardization and healthy reference values.

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