Dataset Information

Effects of dietary probiotic (Pediococcus acidilactici) supplementation on productive performance, egg quality, and body composition in laying hens fed diets varying in energy density.

ABSTRACT: This study was conducted to determine the effect of probiotic Pediococcus acidilactici (PA) strain MA18/5M supplementation of diets with different dietary energy levels on productive performance, egg quality, and body composition in Hy-Line Brown hens during a 16-week period from 32 to 47 wk of age. The experimental treatments with a 2 × 2 factorial design received a 2 wheat-corn-soybean diet: a moderately low energy density diet with 2,650 kcal ME/kg (M-LED) and a low energy density diet based on the M-LED diet with 2,550 kcal ME/kg (LED), each diet without and with probiotic supplementation (M-LED, LED, M-LEDp, and LEDp, respectively). Reduced dietary energy levels had a particularly negative effect on egg weight (61.7 vs. 63.3 g; -2.6%, P < 0.001), egg mass output (1.67 vs. 1.71 kg; -2.4%, P = 0.015), and FCR (2.01 vs. 1.97 kg feed/kg egg; +2%, P = 0.028). In hens administered the LED diet, deteriorated productive performance was accompanied by greater body weight loss (P < 0.001) and reduced abdominal fat content (P < 0.033) as compared with the M-LED group. Dietary probiotic inclusion increased egg weight (P = 0.015), including relative eggshell weight (P = 0.008) and eggshell thickness (P = 0.002) and significantly improved FCR (P = 0.010). No interactions between the PA-based probiotic and dietary energy levels were found in any of the tested parameters. Adding the probiotic on top of the M-LED diet improved layers performance but resulted in nonbioequivalence for the egg weight, egg mass output, and FCR compared with this group without probiotic. Probiotic supplementation of the LEDp diet improved all performance parameters except for egg weight. As a result, the laying rate, egg mass output, daily feed intake, and FCR in the LEDp treatment were bioequivalent to those noted in the M-LED group without the probiotic. The results of a bioequivalence test suggest that a low energy diet fed to laying hens promoted a probiotic response to improve energy utilization by birds.

SUBMITTER: Mikulski D

PROVIDER: S-EPMC7587791 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Effects of dietary probiotic (Pediococcus acidilactici) supplementation on productive performance, egg quality, and body composition in laying hens fed diets varying in energy density.

Mikulski Dariusz D Jankowski Jan J Mikulska Marzena M Demey Vanessa V

Poultry science 20200226 4

This study was conducted to determine the effect of probiotic Pediococcus acidilactici (PA) strain MA18/5M supplementation of diets with different dietary energy levels on productive performance, egg quality, and body composition in Hy-Line Brown hens during a 16-week period from 32 to 47 wk of age. The experimental treatments with a 2 × 2 factorial design received a 2 wheat-corn-soybean diet: a moderately low energy density diet with 2,650 kcal ME/kg (M-LED) and a low energy density diet based ...[more]

PMID: 32241513

Similar Datasets

Project description:A major mean to minimize feeding costs and faecal nitrogen excretion on poultry farms is to decrease the supplied dietary protein content. This, however, is associated with the declines in productive performance and systemic health indices. Sanguinarine may improve protein efficiency via decreasing the intestinal amino acid decarboxylation and stimulating the tryptophan-serotonin pathway. The present study was carried out to investigate the effects of dietary supplementation of sanguinarine on the performance, egg yolk biochemical parameters, serum enzyme activities, nutrient digestibility, ovarian follicles, and hepatic health indices in laying hens fed decremental levels of crude protein (CP). For this purpose, 180 laying hens were allocated into nine dietary treatments with four replicates of five birds each. The experimental treatments consisted of three levels of CP (85.0%, 92.5%, and 100% of Hy-Line W-36 manual recommendation) and three levels of sanguinarine (0.00, 3.75, and 7.50 mg/kg) in a 3 × 3 factorial arrangement administered during a 70-day feeding trial. Results showed that the decremental levels of CP led to significant increases in serum aspartate aminotransferase (p < .05), alanine aminotransferase, and alkaline phosphatase (p < .01) activities, egg yolk cholesterol concentration (p = .064), and hepatic fat and malondialdehyde (MDA) contents (p < .05). It also caused the significant declines in ileal dry matter (DM) digestibility (p < .05) and eggshell strength (p < .05), and also tended to decrease CP digestibility (p = .071), Haugh unit (p = .057) and egg production percentage (p = .062). The interaction effects of the experimental factors indicated that dietary supplementation of sanguinarine, especially at 7.50 mg/kg, led to significant improvements in serum aspartate aminotransferase and alanine aminotransferase activities (p < .01), egg yolk cholesterol (p < .001) and triglyceride (p < .05) concentrations, eggshell strength (p < .001), Haugh unit (p < .05), hepatic fat (p < .001) and MDA (p = .059) contents, ileal DM and CP digestibility (p < .01) as well as egg production, egg mass and feed conversion ratio (FCR; p < .05) in birds receiving decremental levels of CP. Taken together, the results indicate that dietary administration of sanguinarine could enhance productive performance via improving nutrient digestibility, hepatic health indices and fortifying systemic antioxidant capacity in laying hens fed low-CP diets.

Project description:The increasing trend of ban on the use of antibiotic growth promoters (AGPs) across the globe in the poultry industry has led to a growing need for alternatives to AGPs. Prebiotic, probiotic and their combination as a synbiotic have been considered as potential alternatives. This study aimed to investigate the effects of a prebiotic (isomaltooligosaccharide, IMO), a probiotic (PrimaLac®), and their combination (synbiotic) on hen performance, biochemical and haematological responses, and relative organ weights from 20 to 52 weeks of age.Supplementation of 1% IMO (PRE), 0.1% PrimaLac® (PRO) and 1% IMO + 0.1% PrimaLac® (SYN) improved (P < 0.05) feed intake and egg production at 20-36 weeks of age; body weight gain, feed conversion ratio and egg mass at 20-36 and 20-52 weeks of age; and egg weight at 20-36, 37-52 and 20-52 weeks of age. Compared to control-fed hens at 20-36 weeks of age, PRO- and SYN-fed hens produced less (P < 0.05) small size eggs while SYN-fed hens produced more large size eggs. From 37 to 52 weeks of age, PRE-, PRO- or SYN-fed hens produced less (P < 0.05) medium size eggs, and more large and extra-large size eggs. PRE, PRO or SYN supplementation decreased (P < 0.05) the serum total cholesterol at 36 weeks of age, and serum low-density lipoprotein (LDL) cholesterol, alanine aminotransferase (ALT) and alkaline phosphatase (ALP) at 36 and 52 weeks of age. At 36 and 52 weeks of age, supplementation of PRE, PRO or SYN increased (P < 0.05) lymphocyte percentage and decreased (P < 0.05) heterophil percentage, leading to a lower heterophil to lymphocyte (H/L) ratio. No significant differences were observed in the relative weights of the heart, liver, ovary, pancreas and spleen of all dietary treatment groups.Supplementation of PRE, PRO or SYN improved performance, serum total cholesterol, LDL cholesterol, ALT, ALP and H/L ratio of hens from 20 to 52 weeks of age. These results demonstrated the use of PRE, PRO and SYN as alternative feed additives to AGPs for improving the health and productivity of hens, while PRO is the best for commercial layer production to yield maximum profit.

Project description:BackgroundArginine (Arg) is an essential amino acid (EAA) in poultry, an important substrate for protein synthesis and a precursor of several molecules. Supplementation of EAAs with low protein (LP) diet increases the utilization efficiency of dietary crude protein (CP). However, if the EAA requirement is changed in hens fed a LP diet remains to be elucidated. The aim of the present study was to evaluate the optimal level of dietary Arg in the LP diet of hens. A total of 1350 Hy-Line Brown laying hens were randomly allocated to six dietary treatments: a basal diet (16% CP, positive control), or an isoenergetic LP diet (14% CP, 0.80% Arg) supplemented 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg, corresponding to 0.80%, 0.85%, 0.90%, 0.95% and 1.00% dietary Arg, respectively.ResultsThe feed efficiency was decreased (P < 0.05) by 0.80% and 1.00% Arg-LP diets, compared to control. Within LP diets, dietary Arg level had significant quadratic effects (P < 0.05) on laying rate, egg mass, and feed efficiency. Compared to control, the plasma CAT activity or T-AOC content were decreased by 0.80% (P < 0.001). However, the hens offered 0.85% and 0.90% Arg-LP diets had higher CAT activity (P < 0.001) than 0.80% Arg-LP diet. In contrast, 1.00% Arg-LP group had the highest MDA and the lowest T-AOC content in plasma, liver, duodenal and jejunal mucosa (P < 0.05). Compared to control, the villus height was decreased by 0.80%, 0.95% and 1.00% Arg-LP diets, while the villus height to crypt depth (V/C) ratio was reduced by 0.95% and 1.00% Arg-LP diets in duodenum.ConclusionThe result demonstrates that LP diet (14% CP) deficient in Arg (0.80% Arg) result in augmented oxidative damage and impaired development of intestinal mucosa. According to the quadratic broken-line regression model, the optimal dietary arginine levels for Hy-Line Brown laying hens fed with low protein diet (14% CP) aged 33 to 40 weeks are 0.85%, 0.86%, and 0.86% to obtained the maximum laying rate, egg mass, and feed efficiency, respectively.

Project description:The objective of this study is to investigate the beneficial effects and underlying mechanism of dietary β-mannanase supplementation on the productive performance of laying hens fed with metabolic energy (ME)-reduced diets. A total of 448 Hy-Line gray laying hens were randomly assigned to seven groups. Each group had 8 replicates with 8 hens. The groups included a control diet (CON) with a ME of 2750 kcal/Kg, diets reduced by 100 kcal/Kg or 200 kcal/Kg ME (ME_100 or ME_200), and diets with 0.15 g/Kg or 0.2 g/Kg β-mannanase (ME_100+β-M_0.15, ME_100+β-M_0.2, ME_200+β-M_0.15, and ME_200+β-M_0.2). The productive performance, egg quality, intestinal morphology, inflammatory response, mRNA expression related to the Nuclear factor kappa B (NF-κB) and AMPK pathway, and cecum microbiome were evaluated in this study. ME-reduced diets negatively impacted the productive performance of laying hens. However, supplementation with β-mannanase improved FCR, decreased ADFI, and restored average egg weight to the level of the CON group. ME-reduced diets increased the levels of interleukin-1β (IL-1β) and IL-6 while decreasing the levels of IL-4 and IL-10 in the jejunum of laying hens. However, dietary β-mannanase supplementation improved jejunum morphology, reduced pro-inflammatory cytokine concentrations, and increased levels of anti-inflammatory factors in laying hens fed with ME-reduced diets. The mRNA levels of IL-6, IFN-γ, TLR4, MyD88, and NF-κB in the jejunum of ME-reduced diets were significantly higher than that in CON, dietary β-mannanase supplementation decreased these genes expression in laying hens fed with ME-reduced diets. Moreover, dietary β-mannanase supplementation also decreased the mRNA levels of AMPKα and AMPKγ, and increased the abundance of mTOR in the jejunum of laying hens fed with ME-reduced diets. Cecum microbiota analysis revealed that dietary β-mannanase increased the abundance of various beneficial bacteria (e.g., g_Pseudoflavonifractor, g_Butyricicoccus, and f_Lactobacillaceae) in laying hens fed with ME-reduced diets. In conclusion, dietary β-mannanase supplementation could improve the productive performance of laying hens fed with a ME-reduced diet by improving intestinal morphology, alleviating intestinal inflammation, changing energy metabolism-related signaling pathways, and increasing cecum-beneficial microbiota.

Project description:There are an increasing number of studies on the effect of cassava (Manihot esculenta Crantz) on performance of laying hens with inconsistent outcomes. This study, therefore, used a meta-analytic approach to examine the effect of cassava-based diets on feed intake (FI), feed conversion ratio (FCR) end production data such as hen day egg production (HDEP), egg weight (EW), egg mass (EM), Haugh unit (HU), shell weight (SW) and shell thickness (ST) in laying hens. Two hundred and three studies were identified in a search performed in PubMed, Scopus and Google Scholar databases of which thirteen studies were suitable for the meta-analysis. Subgroup and meta-regression analyses were performed to explore the responses of laying hens to dietary cassava and sources of heterogeneity, respectively using the following moderators: study continent, cassava type, cassava processing methods, inclusion level, layer strain, feeding durations, number of layers and hen's age. Data were pooled using a random-effects model and statistical analyses were performed in OpenMEE software. Results show that cassava increased FI (mean difference MD = 0.97 g/d; 95% confidence intervals (CI) 0.05, 1.90) and reduced egg weight (MD = -0.72 g; 95% CI -1.26, -0.19) and SW (MD = -0.11 g; 95% CI -0.18, -0.04) when compared to control. Laying hens fed cassava diets had HDEP, FCR, EM, ST and HU values that compared favourably with the controls. Subgroup analysis demonstrated that inclusion of cassava at ≤ 25% in layer diets had no deleterious effects on measured outcomes taking cognizance of significant heterogeneity. However, meta-regression results showed that most of the sources of heterogeneity were explained by the studied moderators. In conclusion, ≤25% of cassava (CRM and CPM) can be included in layer diets without adverse effects on feed intake, feed conversion ratio and egg production characteristics, indicating that cassava has a very bright future as an energy source in the diets of laying hens.

Dataset Information

Effects of dietary probiotic (Pediococcus acidilactici) supplementation on productive performance, egg quality, and body composition in laying hens fed diets varying in energy density.

Publications

Effects of dietary probiotic (Pediococcus acidilactici) supplementation on productive performance, egg quality, and body composition in laying hens fed diets varying in energy density.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets