Unknown

Dataset Information

0

Restriction of SARS-CoV-2 Replication by Targeting Programmed -1 Ribosomal Frameshifting In Vitro.


ABSTRACT: Translation of open reading frame 1b (ORF1b) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires programmed -1 ribosomal frameshifting (-1 PRF) promoted by an RNA pseudoknot. The extent to which SARS-CoV-2 replication may be sensitive to changes in -1 PRF efficiency is currently unknown. Through an unbiased, reporter-based high-throughput compound screen, we identified merafloxacin, a fluoroquinolone antibacterial, as a -1 PRF inhibitor of SARS-CoV-2. Frameshift inhibition by merafloxacin is robust to mutations within the pseudoknot region and is similarly effective on -1 PRF of other beta coronaviruses. Importantly, frameshift inhibition by merafloxacin substantially impedes SARS-CoV-2 replication in Vero E6 cells, thereby providing the proof of principle of targeting -1 PRF as an effective antiviral strategy for SARS-CoV-2.

SUBMITTER: Sun Y 

PROVIDER: S-EPMC7587830 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8256030 | biostudies-literature
| S-EPMC7111862 | biostudies-literature
2022-12-22 | GSE206101 | GEO
| S-EPMC9884621 | biostudies-literature
| S-EPMC8923105 | biostudies-literature
| S-EPMC6787027 | biostudies-literature
| S-EPMC8310280 | biostudies-literature
| S-EPMC8664833 | biostudies-literature
| S-EPMC8353986 | biostudies-literature
2024-07-11 | GSE223690 | GEO