Combining impedance biosensor with immunomagnetic separation for rapid screening of Salmonella in poultry supply chains.

ABSTRACT: Salmonella screening is a key to ensure food safety in poultry supply chains. Currently available Salmonella detection methods including culture, polymerase chain reaction and enzyme-linked immuno-sorbent assay could not achieve rapid, sensitive, and in-field detection. In this study, different strategies for separation and detection of Salmonella were proposed, compared, and improved based on our previous studies on immunomagnetic separation and impedance biosensor. First, the coaxial capillary for immunomagnetic separation of target bacteria was improved with less contamination, and 3 strategies based on the improved capillary and immunomagnetic nanoparticles were compared to separate the target bacteria from sample and form the magnetic bacteria. The experimental results showed that the

SUBMITTER: Wang L 

PROVIDER: S-EPMC7587860 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature