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Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming.


ABSTRACT: This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-?) and gap junctions (CX43) in oocytes and their derived blastocysts. Vitrification gave rise to abnormal spindle microtubule configurations and elevated ROS levels. Supplementation of IVM medium with GSH-OEt before vitrification preserved mitochondrial distribution pattern and diminished both cytoplasmic and mitochondrial ROS contents and percentages of embryos developing beyond the 8-cell stage were similar to those recorded in fresh non-vitrified oocytes. Although not significantly different from control vitrified oocytes, vitrified oocytes after GSH-OEt treatment gave rise to similar day 8-blastocyst and hatching rates to fresh non-vitrified oocytes. No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of GPX1, AQP3 and CX43 in derived blastocysts. The addition of GSH-OEt to the IVM medium before vitrification may be beneficial for embryo development presumably as the consequence of additional anti-oxidant protection during IVM.

SUBMITTER: Garcia-Martinez T 

PROVIDER: S-EPMC7588878 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Glutathione Ethyl Ester Protects In Vitro<i>-</i>Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming.

García-Martínez Tania T   Vendrell-Flotats Meritxell M   Martínez-Rodero Iris I   Ordóñez-León Erika Alina EA   Álvarez-Rodríguez Manuel M   López-Béjar Manel M   Yeste Marc M   Mogas Teresa T  

International journal of molecular sciences 20201013 20


This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (<i>BAX, BCL2</i>), oxidative-stress pathways (<i>GPX1, SOD  ...[more]

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2022-04-02 | GSE133550 | GEO