A Novel HPLC-Based Method to Investigate on RNA after Fixation.
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ABSTRACT:
SUBMITTER: Fattorini P
PROVIDER: S-EPMC7588918 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
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A Novel HPLC-Based Method to Investigate on RNA after Fixation.
Fattorini Paolo P Forzato Cristina C Tierno Domenico D De Martino Eleonora E Azzalini Eros E Canzonieri Vincenzo V Stanta Giorgio G Bonin Serena S
International journal of molecular sciences 20201013 20
RNA isolated from fixed and paraffin-embedded tissues is widely used in biomedical research and molecular pathology for diagnosis. In the present study, we have set-up a method based on high performance liquid chromatography (HPLC) to investigate the effects of different fixatives on RNA. By the application of the presented method, which is based on the Nuclease S1 enzymatic digestion of RNA extracts followed by a HPLC analysis, it is possible to quantify the unmodified nucleotide monophosphates ...[more]
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