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Tetrahydroimidazo[1,2-a]pyrazine Derivatives: Synthesis and Evaluation as Gαq -Protein Ligands.

ABSTRACT: The 5,6,7,8-tetrahydroimidazo[1,2-a]pyrazine derivative BIM-46174 and its dimeric form BIM-46187 (1) are heterocyclized dipeptides that belong to the very few cell-permeable compounds known to preferentially silence Gαq proteins. To explore the chemical space of Gαq inhibitors of the BIM chemotype, a combinatorial approach was conducted towards a library of BIM molecules. This library was evaluated in a second messenger-based fluorescence assay to analyze the activity of Gαq proteins through the determination of intracellular myo-inositol 1-phosphate. Structure-activity relationships were deduced and structural requirements for biological activity obtained, which were (i) a redox reactive thiol/disulfane substructure, (ii) an N-terminal basic amino group, (iii) a cyclohexylalanine moiety, and (iv) a bicyclic skeleton. Active compounds exhibited cellular toxicity, which was investigated in detail for the prototypical inhibitor 1. This compound affects the structural cytoskeletal dynamics in a Gαq/11 -independent manner.

SUBMITTER: Kuppers J 

PROVIDER: S-EPMC7590114 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Tetrahydroimidazo[1,2-a]pyrazine Derivatives: Synthesis and Evaluation as Gα<sub>q</sub> -Protein Ligands.

Küppers Jim J   Benkel Tobias T   Annala Suvi S   Kimura Kenichi K   Reinelt Lisa L   Fleischmann Bernd K BK   Kostenis Evi E   Gütschow Michael M  

Chemistry (Weinheim an der Bergstrasse, Germany) 20200907 55

The 5,6,7,8-tetrahydroimidazo[1,2-a]pyrazine derivative BIM-46174 and its dimeric form BIM-46187 (1) are heterocyclized dipeptides that belong to the very few cell-permeable compounds known to preferentially silence Gα<sub>q</sub> proteins. To explore the chemical space of Gα<sub>q</sub> inhibitors of the BIM chemotype, a combinatorial approach was conducted towards a library of BIM molecules. This library was evaluated in a second messenger-based fluorescence assay to analyze the activity of Gα  ...[more]

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