Dataset Information

Enhancing ?-Carotene Concentration in Parental Lines of CO6 Maize Hybrid Through Marker-Assisted Backcross Breeding (MABB).

ABSTRACT: Vitamin A deficiency (VAD) is a global health problem; many people around the world, especially children and pregnant women, are VAD deficient or insufficient. Maize is known as an important source of provitamin A for humans. Hence, enhancement of provitamin A carotenoids (pVAC) in maize varieties through breeding or biofortification is a good option for alleviating VAD in developing countries, especially India. So far, numerous maize hybrids have been developed in India. Among them, CO6, derived from UMI1200 × UMI1230, is a popular maize hybrid and adapted to different agro-climatic zones of India, especially Tamil Nadu, a southern state of India. However, CO6 is deficient for pVAC carotenoid ?-carotene. Thus, the objectives of this study were to increase the ?-carotene concentration in UMI1200 and UMI1230 and generate the ?-carotene enriched hybrids through marker-assisted backcross breeding (MABB). For this purpose, the maize genotype HP467-15 was used as the donor for transferring the ?-carotene gene, crtRB1, into UMI1200 and UMI1230. In the MABB scheme, we used one gene-specific marker (crtRB1 3'TE) and 214 simples sequence repeat (SSR) markers for foreground and background selection, respectively. As a result, six improved lines with recurrent parent genome recovery (RPGR) ranging from 90.24 to 92.42%, along with good agronomic performance, were generated. The ?-carotene concentration of the improved lines ranged from 7.056 to 9.232 ?g/g. Furthermore, five hybrid combinations were generated using improved lines and evaluated in a comparative yield trial (CYT) and multi-location trials (MLT) along with the original hybrid CO6 and commercial hybrids. It was revealed that ACM-M13-002 was a superior hybrid with a 7.3-fold increase in ?-carotene concentration and with a comparable yield to CO6. In summary, the improved maize inbreds can be used as possible donors for the development of ?-carotene-rich cultivars in maize breeding programs and the ?-carotene enriched hybrid developed in this study will hold great promise for food and nutritional security.

SUBMITTER: Natesan S

PROVIDER: S-EPMC7591750 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Enhancing β-Carotene Concentration in Parental Lines of CO6 Maize Hybrid Through Marker-Assisted Backcross Breeding (MABB).

Natesan Senthil S Duraisamy Thirusenduraselvi T Pukalenthy Bharathi B Chandran Sarankumar S Nallathambi Jagadeeshselvam J Adhimoolam Karthikeyan K Manickam Dhasarathan D Sampathrajan Vellaikumar V Muniyandi Samuel Jeberson SJ Meitei Laishram Joykumar LJ Thirunavukkarasu Nepolean N Kalipatty Nalliappan Ganesan G Rajasekaran Ravikesavan R

Frontiers in nutrition 20201014

Vitamin A deficiency (VAD) is a global health problem; many people around the world, especially children and pregnant women, are VAD deficient or insufficient. Maize is known as an important source of provitamin A for humans. Hence, enhancement of provitamin A carotenoids (pVAC) in maize varieties through breeding or biofortification is a good option for alleviating VAD in developing countries, especially India. So far, numerous maize hybrids have been developed in India. Among them, CO6, derive ...[more]

PMID: 33154974

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Dataset Information

Enhancing ?-Carotene Concentration in Parental Lines of CO6 Maize Hybrid Through Marker-Assisted Backcross Breeding (MABB).

Publications

Enhancing β-Carotene Concentration in Parental Lines of CO6 Maize Hybrid Through Marker-Assisted Backcross Breeding (MABB).

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