Dataset Information

In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom.

ABSTRACT: Contact with stinging spines venom from several Lepidoptera larvae may result in skin lesions. In Mexico, envenomation outbreaks caused by Megalopyge opercularis were reported between 2015 and 2016. The aim of this study was to identify the venomous caterpillars in Nuevo Leon, Mexico and evaluate several biological activities of their hemolymph (HEV) and spine setae (SSV) venoms. M. opercularis was identified by cytochrome oxidase subunit (COI) designed primers. HEV and SSV extracts cytotoxic activity was assessed on the L5178Y-R lymphoma cell line. For apoptotic cells number and apoptosis, cells were stained with acridine orange/ethidium bromide and validated by DNA fragmentation. Human peripheral blood mononuclear cells (hPBMC) cytokine response to the extracts was measured by the cytometric bead array assay. Extracts effect on pro-coagulation activity on human plasma was also evaluated. HEV and SSV extracts significantly inhibited (p?

SUBMITTER: Orozco-Flores AA

PROVIDER: S-EPMC7592054 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom.

Orozco-Flores Alonso A AA Valadez-Lira José A JA Covarrubias-Cárdenas Karina E KE Pérez-Trujillo José J JJ Gomez-Flores Ricardo R Caballero-Hernández Diana D Tamez-Guerra Reyes R Rodríguez-Padilla Cristina C Tamez-Guerra Patricia P

Scientific reports 20201027 1

Contact with stinging spines venom from several Lepidoptera larvae may result in skin lesions. In Mexico, envenomation outbreaks caused by Megalopyge opercularis were reported between 2015 and 2016. The aim of this study was to identify the venomous caterpillars in Nuevo Leon, Mexico and evaluate several biological activities of their hemolymph (HEV) and spine setae (SSV) venoms. M. opercularis was identified by cytochrome oxidase subunit (COI) designed primers. HEV and SSV extracts cytotoxic ac ...[more]

PMID: 33110124

Similar Datasets

Project description:In an emergency response to the introduction, subsequent detection and rapid spread of the invasive insect pest fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) in the country, Government of India offered ad-hoc approval for few pesticide molecules namely, emamectin benzoate, spinetoram, chlorantraniliprole, novaluron, thiodicarb, and λ-cyhalothrin for FAW management in corn crop across the country. Five major maize (corn) growing geographical areas (i.e., Bihar, Delhi, Karnataka, Punjab and Tamil Nadu) were selected during the main crop season of 2020 (Oct-Nov), and sampled for the target-insect populations. The insect populations were lab-reared on maize leaves (15-20 days old); the F1 generation insects (third instar, 25-30 mg/larva) were subjected to bioassay to determine susceptibility levels of FAW against ad-hoc recommended insecticides. The previously reported target-site molecular variations in the genes ace, encoding acetylcholinesterase (AChE), and vgsc, encoding voltage-gated sodium channel were analyzed. Among the five test-populations, Bihar test-population recorded least susceptibility to all the test-pesticides, whereas the South Indian populations (Karnataka, Tamil Nadu) were found most susceptible. North Indian and South Indian test-insect populations formed two distinct groups in terms of susceptibility levels, speculatively on account of prevailing climatic factors. Being the population with least ace mutation frequency, but with the higher resistance ratio for all the test-pesticides, Bihar insect population implies a bigger role of broad range detoxification machinery than the narrow scope of target site insensitivity. Though, resistance has not developed to the recommended insecticides by FAW, except the case of low-medium resistance development; which is better explained due to behavioural avoidance of synthetic pyrethroid (λ-cyhalothrin). However, there is no room for complacency. Resistance-monitoring tools such as location/region-specific determination of discriminating diagnostic concentrations/doses for FAW in recommended insecticides are to be devised at the earliest.Supplementary informationThe online version contains supplementary material available at 10.1007/s13205-022-03303-2.

Dataset Information

In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom.

Publications

In vitro antitumor, pro-inflammatory, and pro-coagulant activities of Megalopyge opercularis J.E. Smith hemolymph and spine venom.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets