Unknown

Dataset Information

0

Qualitative Differences Between the IFN? subtypes and IFN? Influence Chronic Mucosal HIV-1 Pathogenesis.


ABSTRACT: The Type I Interferons (IFN-Is) are innate antiviral cytokines that include 12 different IFN? subtypes and IFN? that signal through the IFN-I receptor (IFNAR), inducing hundreds of IFN-stimulated genes (ISGs) that comprise the 'interferome'. Quantitative differences in IFNAR binding correlate with antiviral activity, but whether IFN-Is exhibit qualitative differences remains controversial. Moreover, the IFN-I response is protective during acute HIV-1 infection, but likely pathogenic during the chronic stages. To gain a deeper understanding of the IFN-I response, we compared the interferomes of IFN? subtypes dominantly-expressed in HIV-1-exposed plasmacytoid dendritic cells (1, 2, 5, 8 and 14) and IFN? in the earliest cellular targets of HIV-1 infection. Primary gut CD4 T cells from 3 donors were treated for 18 hours ex vivo with individual IFN-Is normalized for IFNAR signaling strength. Of 1,969 IFN-regulated genes, 246 'core ISGs' were induced by all IFN-Is tested. However, many IFN-regulated genes were not shared between the IFN? subtypes despite similar induction of canonical antiviral ISGs such as ISG15, RSAD2 and MX1, formally demonstrating qualitative differences between the IFN? subtypes. Notably, IFN? induced a broader interferome than the individual IFN? subtypes. Since IFN?, and not IFN?, is upregulated during chronic HIV-1 infection in the gut, we compared core ISGs and IFN?-specific ISGs from colon pinch biopsies of HIV-1-uninfected (n = 13) versus age- and gender-matched, antiretroviral-therapy naïve persons with HIV-1 (PWH; n = 19). Core ISGs linked to inflammation, T cell activation and immune exhaustion were elevated in PWH, positively correlated with plasma lipopolysaccharide (LPS) levels and gut IFN? levels, and negatively correlated with gut CD4 T cell frequencies. In sharp contrast, IFN?-specific ISGs linked to protein translation and anti-inflammatory responses were significantly downregulated in PWH, negatively correlated with gut IFN? and LPS, and positively correlated with plasma IL6 and gut CD4 T cell frequencies. Our findings reveal qualitative differences in interferome induction by diverse IFN-Is and suggest potential mechanisms for how IFN? may drive HIV-1 pathogenesis in the gut.

SUBMITTER: Guo K 

PROVIDER: S-EPMC7592919 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications


The Type I Interferons (IFN-Is) are innate antiviral cytokines that include 12 different IFNα subtypes and IFNβ that signal through the IFN-I receptor (IFNAR), inducing hundreds of IFN-stimulated genes (ISGs) that comprise the 'interferome'. Quantitative differences in IFNAR binding correlate with antiviral activity, but whether IFN-Is exhibit qualitative differences remains controversial. Moreover, the IFN-I response is protective during acute HIV-1 infection, but likely pathogenic during the c  ...[more]

Similar Datasets

| S-EPMC10951235 | biostudies-literature
| S-EPMC7145903 | biostudies-literature
| S-EPMC7614897 | biostudies-literature
| S-EPMC4157516 | biostudies-literature
| S-EPMC3164950 | biostudies-literature
| S-EPMC5428457 | biostudies-literature
| S-EPMC5641679 | biostudies-literature
| S-EPMC2631133 | biostudies-other
| S-EPMC3598776 | biostudies-literature
| S-EPMC9616162 | biostudies-literature