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Genotyping of methicillin resistant Staphylococcus aureus from the United Arab Emirates.

ABSTRACT: Reports from Arabian Gulf countries have demonstrated emergence of novel methicillin resistant Staphylococcus aureus (MRSA) strains. To address the lack of data from the United Arab Emirates (UAE), genetic characterisation of MRSA identified between December 2017 and August 2019 was conducted using DNA microarray-based assays. The 625 MRSA isolates studied were grouped into 23 clonal complexes (CCs) and assigned to 103 strains. CC5, CC6, CC22 and CC30 represented 54.2% (n/N?=?339/625) of isolates with other common CCs being CC1, CC8, CC772, CC361, CC80, CC88. Emergence of CC398 MRSA, CC5-MRSA-IV Sri Lanka Clone and ST5/ST225-MRSA-II, Rhine-Hesse EMRSA/New York-Japan Clone in our setting was detected. Variants of pandemic CC8-MRSA-[IVa?+?ACME I] (PVL+) USA300 were detected and majority of CC772 strains were CC772-MRSA-V (PVL+), "Bengal- Bay Clone". Novel MRSA strains identified include CC5-MRSA-V (edinA+), CC5-MRSA-[VT?+?fusC], CC5-MRSA-IVa (tst1+), CC5-MRSA-[V/VT?+?cas?+?fusC?+?ccrA/B-1], CC8-MRSA-V/VT, CC22-MRSA-[IV?+?fusC?+?ccrAA/(C)], CC45-MRSA-[IV?+?fusC?+?tir], CC80-MRSA-IVa, CC121-MRSA-V/VT, CC152-MRSA-[V?+?fusC] (PVL+). Although several strains harboured SCC-borne fusidic acid resistance (fusC) (n?=?181), erythromycin/clindamycin resistance (ermC) (n?=?132) and gentamicin resistance (aacA-aphD) (n?=?179) genes, none harboured vancomycin resistance genes while mupirocin resistance gene mupR (n?=?2) and cfr gene (n?=?1) were rare. An extensive MRSA repertoire including CCs previously unreported in the region and novel strains which probably arose locally suggest an evolving MRSA landscape.

SUBMITTER: Senok A

PROVIDER: S-EPMC7596093 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Genotyping of methicillin resistant Staphylococcus aureus from the United Arab Emirates.

Senok Abiola A Nassar Rania R Celiloglu Handan H Nabi Anju A Alfaresi Mubarak M Weber Stefan S Rizvi Irfan I Müller Elke E Reissig Annett A Gawlik Darius D Monecke Stefan S Ehricht Ralf R

Scientific reports 20201029 1

Reports from Arabian Gulf countries have demonstrated emergence of novel methicillin resistant Staphylococcus aureus (MRSA) strains. To address the lack of data from the United Arab Emirates (UAE), genetic characterisation of MRSA identified between December 2017 and August 2019 was conducted using DNA microarray-based assays. The 625 MRSA isolates studied were grouped into 23 clonal complexes (CCs) and assigned to 103 strains. CC5, CC6, CC22 and CC30 represented 54.2% (n/N = 339/625) of isolate ...[more]

PMID: 33122734

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Project description:BACKGROUND:Globally, methicillin resistant Staphylococcus aureus (MRSA) is one of the most common pathogens that causes hospital- and community-acquired infections. The use of molecular typing methods is essential for determining the origin of the isolates, their clonal relations, and also epidemiological investigations. OBJECTIVE:The purpose of this study was to determine the prevalence of antibiotic-resistant MRSA investigate the accessory gene regulator (agr) and staphylococcal cassette chromosome mec (SCCmec) types and perform multilocus sequence typing (MLST). Furthermore, the minimum inhibitory concentration of MRSA isolates was determined for vancomycin and daptomycin. MATERIALS AND METHODS:Two hundred and fifty-nine MRSA isolates were collected from Tertiary Care Hospitals in Coimbatore. Disk diffusion method was employed to assess the sensitivity of MRSA isolates to selected antibiotics and genetic analysis was performed using SCCmec, agr, and MLST typing by multiplex-polymerase chain reaction strategy. Minimal inhibitory concentration (MIC) was determined using Ezy MIC (vancomycin) and Biomerieux (daptomycin) E-test strip. RESULTS:Of 259 MRSA isolates, 209 (80.7%) were confirmed as methicillin resistant. Antibiotic susceptibility pattern revealed that all the MRSA isolates were 100% sensitive to linezolid, rifampicin, teicoplanin, and vancomycin. MIC results showed that of 209 MRSA isolates, 10 were found to be vancomycin intermediate S. aureus and 100% of the MRSA isolates were daptomycin-susceptible. The agr group I and SCCmec Type III were the major type among MRSA isolates. In addition to these MLST typing revealed the prevalence of sequence type (ST) 239 (SLV of ST8) among the MRSA isolates. CONCLUSION:This study confirms that ST239 (Brazilian clone) of MRSA is predominant in this region which is responsible for the hospital-acquired MRSA infections. Thus, the study also suggests that vancomycin and daptomycin can still be used as an alternative drug for treating severe MRSA infections.

Dataset Information

Genotyping of methicillin resistant Staphylococcus aureus from the United Arab Emirates.

Publications

Genotyping of methicillin resistant Staphylococcus aureus from the United Arab Emirates.

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