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Effects of selenide chitosan sulfate on glutathione system in hepatocytes and specific pathogen-free chickens.

ABSTRACT: This study aimed to investigate the effects of selenide chitosan sulfate (Se-CTS-S) on glutathione (GSH) system in hepatocytes and chickens. Chitosan, sodium selenite (Na2SeO3), selenide chitosan, chitosan sulfate (CTS-S), and Se-CTS-S were added to the culture medium and the basal diets; glutathione peroxidase (GSH-Px) activity, GSH content, total antioxidant capacity (T-AOC), and mRNA levels of cellular GPx (GPx-1) and phospholipid hydroperoxide GPx (GPx-4) in vivo and in vitro were determined. The results showed that Se-CTS-S increased (P < 0.05) GPx-1 and GPx-4 mRNA levels in hepatocytes and livers, and GSH-Px activity, GSH content, and T-AOC in the medium, hepatocytes, plasma, and livers compared with the control and chitosan treatments. Compared with CTS-S, Se-CTS-S treatments increased (P < 0.05) GPx-1 and GPx-4 mRNA levels in hepatocytes and livers, and GSH-Px activity, GSH content, and T-AOC capacity in the medium, hepatocytes, and livers. Compared with Na2SeO3 and CTS-Se, Se-CTS-S increased (P < 0.05) GPx-1 mRNA levels in hepatocytes and livers, GPx-4 mRNA levels in hepatocytes and livers, GSH-Px activity in the medium, hepatocytes, and livers, GSH contents in plasma and livers, and T-AOC in the medium, plasma, and livers. Thus, Se-CTS-S showed better biological activity that mainly benefited from the synergistic effects of Se and sulfate on GSH system.

SUBMITTER: Chen F

PROVIDER: S-EPMC7597923 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Effects of selenide chitosan sulfate on glutathione system in hepatocytes and specific pathogen-free chickens.

Chen Fu F Hou Lele L Zhu Lianqin L ChengboYang Zhu Fenghua F Qiu Huiling H Qin Shunyi S

Poultry science 20200526 8

This study aimed to investigate the effects of selenide chitosan sulfate (Se-CTS-S) on glutathione (GSH) system in hepatocytes and chickens. Chitosan, sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>), selenide chitosan, chitosan sulfate (CTS-S), and Se-CTS-S were added to the culture medium and the basal diets; glutathione peroxidase (GSH-Px) activity, GSH content, total antioxidant capacity (T-AOC), and mRNA levels of cellular GPx (GPx-1) and phospholipid hydroperoxide GPx (GPx-4) in vivo and in ...[more]

PMID: 32731985

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Dataset Information

Effects of selenide chitosan sulfate on glutathione system in hepatocytes and specific pathogen-free chickens.

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Effects of selenide chitosan sulfate on glutathione system in hepatocytes and specific pathogen-free chickens.

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