Dataset Information

Transcriptomic and metabolomic profiling of melatonin treated soybean (Glycine max L.) under drought stress during grain filling period through regulation of secondary metabolite biosynthesis pathways.

ABSTRACT: There is a growing need to enhance the productivity of soybean (Glycine max L.) under severe drought conditions in order to improve global food security status. Melatonin, a ubiquitous hormone, could alleviate drought stress in various plants. Earlier, we demonstrated that exogenous melatonin treatment could enhance the tolerance of drought-treated soybean. However, the underlying mechanisms by which this hormone exerts drought resistance is still unclear. The present study used transcriptomic and metabolomic techniques to determine some critical genes and pathways regulating melatonin response to drought conditions. Results showed that exogenous melatonin treatment could increase relative water content and decrease electrolyte leakage in the leaves and increase seed yield under drought stress. Transcriptomic analysis showed that there were 852 core differentially expressed genes (DEGs) that were regulated by drought stress and melatonin in soybean leaves. The most enriched drought-responsive genes are mainly involved in the 'biosynthesis of secondary metabolites'. Metabolomic profiling under drought stress showed higher accumulation levels of secondary metabolites related to drought tolerance after exogenous melatonin treatment. Also, we highlighted the vital role of the pathways including phenylpropanoid, flavonoid, isoflavonoid, and steroid biosynthesis pathways for improvement of drought tolerance in soybean by exogenous melatonin treatment. In all, findings from this study give detailed molecular basis for the application of melatonin as a drought-resistant agent in soybean cultivation.

SUBMITTER: Cao L

PROVIDER: S-EPMC7598510 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Transcriptomic and metabolomic profiling of melatonin treated soybean (Glycine max L.) under drought stress during grain filling period through regulation of secondary metabolite biosynthesis pathways.

Cao Liang L Jin Xijun X Zhang Yuxian Y Zhang Mingcong M Wang Yanhong Y

PloS one 20201030 10

There is a growing need to enhance the productivity of soybean (Glycine max L.) under severe drought conditions in order to improve global food security status. Melatonin, a ubiquitous hormone, could alleviate drought stress in various plants. Earlier, we demonstrated that exogenous melatonin treatment could enhance the tolerance of drought-treated soybean. However, the underlying mechanisms by which this hormone exerts drought resistance is still unclear. The present study used transcriptomic a ...[more]

PMID: 33125378

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Project description:Heat stress is a primary constraint to Australia's barley production. In addition to impacting grain yield, it adversely affects physical grain quality (weight and plumpness) and market value. The incidence of heat stress during grain filling is rising with global warming. However, breeding for new superior heat-tolerant genotypes has been challenging due to the narrow window of sensitivity, the unpredictable nature of heat stress, and its frequent co-occurrence with drought stress. Greater scientific knowledge regarding traits and mechanisms associated with heat tolerance would help develop more efficient selection methods. Our objective was to assess 157 barley varieties of contrasting genetic backgrounds for various developmental, agro-morphological, and physiological traits to examine the effects of heat stress on physical grain quality. Delayed sowing (i.e., July and August) increased the likelihood of daytime temperatures above 30°C during grain-filling. Supplementary irrigation of field trials ensured a reduced impact of drought stress. Heat tolerance appeared to be the primary factor determining grain plumpness. A wide variation was observed for heat tolerance, particularly among the Australian varieties. Genotypic variation was also observed for grain weight, plumpness, grain growth components, stay-green and stem water-soluble carbohydrates (WSC) content, and mobilisation under normal and delayed sown conditions. Compared to normal sowing, delayed sowing reduced duration of developmental phases, plant height, leaf size, head length, head weight, grain number, plumpness, grain width and thickness, stem WSC content, green leaf area retention, and harvest index (HI), and increased screenings, grain length, grain-filling rate (GFR), WSC mobilisation efficiency (WSCME), and grain protein content. Overall, genotypes with heavier and plumper grains under high temperatures had higher GFR, longer grain-filling duration, longer green leaf area retention, higher WSCME, taller stature, smaller leaf size, greater HI, higher grain weight/plumpness potentials, and earlier flowering. GFR played a significant role in determining barley grain weight and plumpness under heat-stress conditions. Enhancing GFR may provide a new avenue for improving heat tolerance in barley.

Dataset Information

Transcriptomic and metabolomic profiling of melatonin treated soybean (Glycine max L.) under drought stress during grain filling period through regulation of secondary metabolite biosynthesis pathways.

Publications

Transcriptomic and metabolomic profiling of melatonin treated soybean (Glycine max L.) under drought stress during grain filling period through regulation of secondary metabolite biosynthesis pathways.

Similar Datasets

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